IL-23 modulates histamine-evoked itch and responses of pruriceptors in mice

Darya Pavlenko, Hideki Funahashi, Kent Sakai, Takashi Hashimoto, Taisa Lozada, Gil Yosipovitch, Tasuku Akiyama

Research output: Contribution to journalArticlepeer-review

Abstract

Accumulating evidence has highlighted the essential roles of cytokines in itch processing. Although IL-23 and Th17 cytokines are elevated in inflammatory skin disorders, their role in itch is unknown. Here, we investigated the role of IL-23 and IL-17A in itch response using an in vitro calcium imaging of mouse dorsal root ganglion (DRG) neurons and an in vivo behaviour test. Calcium imaging studies revealed that a few DRG neurons (~5%) responded to either IL-23 or IL-17A. Pretreatment cells with IL-23 significantly reduced calcium responses to histamine and capsaicin but not chloroquine. Behaviour experiments showed neither IL-23 nor IL-17A evoked scratching. IL-23 significantly decreased histamine-evoked scratching without affecting chloroquine-evoked scratching. There was no difference in scratching between IL-17A- and vehicle-treated groups. These results indicate that IL-23 might play a role in regulating histaminergic itch via modulation of TRPV1 activity.

Original languageEnglish (US)
JournalExperimental dermatology
DOIs
StateAccepted/In press - 2020

Keywords

  • chloroquine
  • cytokines
  • DRG
  • IL-17
  • pruritus
  • scratching

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Fingerprint Dive into the research topics of 'IL-23 modulates histamine-evoked itch and responses of pruriceptors in mice'. Together they form a unique fingerprint.

Cite this