IL-2 during in vitro priming promotes subsequent engraftment and successful adoptive tumor immunotherapy by persistent memory phenotypic CD8+ T cell

O. F. Bathe, N. Dalyot-Herman, Thomas Malek

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Adoptive T cell tumor immunotherapy potentially consists of two protective components by the transferred effector cells, the immediate immune response and the subsequent development of memory T cells. The extent by which adoptively transferred CD8+ CTL are destined to become memory T cells is ambiguous as most studies focus on the acute effects on tumor shortly following adoptive transfer. In this study we show that a substantial fraction of the input CTL develop into memory cells that reject a s.c. tumor challenge. The use of exogenous IL-2 or a combination of IL-2 and IL-4, but not solely IL-4, during the ex vivo culture for the CTL inoculation was necessary for efficient development of CD8+ memory T cells. Thus, an important component of adoptive immunotherapy using CTL is the production of CD8+ Ag-specific memory cells which is primarily favored by IL-2 receptor signaling during ex vivo generation of the effector CTL.

Original languageEnglish
Pages (from-to)4511-4517
Number of pages7
JournalJournal of Immunology
Volume167
Issue number8
StatePublished - Oct 15 2001

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Adoptive Immunotherapy
Interleukin-2
T-Lymphocytes
Interleukin-4
Neoplasms
Adoptive Transfer
Interleukin-2 Receptors
Immunotherapy
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

IL-2 during in vitro priming promotes subsequent engraftment and successful adoptive tumor immunotherapy by persistent memory phenotypic CD8+ T cell. / Bathe, O. F.; Dalyot-Herman, N.; Malek, Thomas.

In: Journal of Immunology, Vol. 167, No. 8, 15.10.2001, p. 4511-4517.

Research output: Contribution to journalArticle

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