IL-10 production is enhanced in human T cells by IL-12 and IL-6 and in monocytes by tumor necrosis factor-α

Pirouz M. Daftarian, Ashok Kumar, Marko Kryworuchko, Francisco Diaz-Mitoma

Research output: Contribution to journalArticle

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Abstract

IL-10, an immunoregulatory cytokine produced by T cells and monocytes, inhibits the expression of inflammatory and hemopoietic cytokines as well as its own expression. To evaluate the regulation of IL-10 production by T cells and monocytes, we measured IL-10 levels by ELISA in supernatants of PHA- stimulated PBMC following depletion of either T cells or monocytes. IL-10 production was significantly down-regulated in both T cell- and monocyte- depleted PBMC compared with undepleted PBMC, and IL-10 production could be restored by the addition of monocyte-conditioned medium (supernatant of PHA- stimulated, T cell-depleted PBMC), suggesting that IL-10 production by T cells is regulated by a monokine(s) produced by activated monocytes. To further clarify the monokine(s) responsible for IL-10 induction, we stimulated monocyte-depleted PBMC, purified CD4 +, and CD8 + T cells with PHA and measured IL-10 production by ELISA and semiquantitative reverse transcriptase-PCR following monokine(s) addition. Addition of IL-6 and IL-12 enhanced IL-10 production in monocyte-depleted PBMC in a dose-dependent and additive manner. Furthermore, anti-IL-6 and anti-IL-12 Abs neutralized the IL-10-inductive effect of monocyte-conditioned medium. Similarly, IL-12 and IL-6 induced IL-10 production by purified CD4 + and CD8 + T cells. With respect to regulation of IL-10 produced by monocytes, TNF-α was found to induce IL-10 production by resting as well as by LPS-stimulated purified monocytes/macrophages. Taken together, these findings suggest that IL-10 production by human T cells and monocytes is differentially regulated. IL-12 and/or IL-6 can induce the expression of IL-10 by PHA-stimulated T cells, whereas TNF-α induces IL-10 production by monocytes. Since IL-10 inhibits the production of IL-6, IL-12, and TNF-α, these results may indicate a potential mechanism of negative feedback regulation of the immune response.

Original languageEnglish (US)
Pages (from-to)12-20
Number of pages9
JournalJournal of Immunology
Volume157
Issue number1
StatePublished - Jul 1 1996
Externally publishedYes

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Interleukin-12
Interleukin-10
Monocytes
Interleukin-6
Tumor Necrosis Factor-alpha
T-Lymphocytes
Monokines
Conditioned Culture Medium
Enzyme-Linked Immunosorbent Assay
Cytokines
Reverse Transcriptase Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

Cite this

Daftarian, P. M., Kumar, A., Kryworuchko, M., & Diaz-Mitoma, F. (1996). IL-10 production is enhanced in human T cells by IL-12 and IL-6 and in monocytes by tumor necrosis factor-α. Journal of Immunology, 157(1), 12-20.

IL-10 production is enhanced in human T cells by IL-12 and IL-6 and in monocytes by tumor necrosis factor-α. / Daftarian, Pirouz M.; Kumar, Ashok; Kryworuchko, Marko; Diaz-Mitoma, Francisco.

In: Journal of Immunology, Vol. 157, No. 1, 01.07.1996, p. 12-20.

Research output: Contribution to journalArticle

Daftarian, PM, Kumar, A, Kryworuchko, M & Diaz-Mitoma, F 1996, 'IL-10 production is enhanced in human T cells by IL-12 and IL-6 and in monocytes by tumor necrosis factor-α', Journal of Immunology, vol. 157, no. 1, pp. 12-20.
Daftarian, Pirouz M. ; Kumar, Ashok ; Kryworuchko, Marko ; Diaz-Mitoma, Francisco. / IL-10 production is enhanced in human T cells by IL-12 and IL-6 and in monocytes by tumor necrosis factor-α. In: Journal of Immunology. 1996 ; Vol. 157, No. 1. pp. 12-20.
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AB - IL-10, an immunoregulatory cytokine produced by T cells and monocytes, inhibits the expression of inflammatory and hemopoietic cytokines as well as its own expression. To evaluate the regulation of IL-10 production by T cells and monocytes, we measured IL-10 levels by ELISA in supernatants of PHA- stimulated PBMC following depletion of either T cells or monocytes. IL-10 production was significantly down-regulated in both T cell- and monocyte- depleted PBMC compared with undepleted PBMC, and IL-10 production could be restored by the addition of monocyte-conditioned medium (supernatant of PHA- stimulated, T cell-depleted PBMC), suggesting that IL-10 production by T cells is regulated by a monokine(s) produced by activated monocytes. To further clarify the monokine(s) responsible for IL-10 induction, we stimulated monocyte-depleted PBMC, purified CD4 +, and CD8 + T cells with PHA and measured IL-10 production by ELISA and semiquantitative reverse transcriptase-PCR following monokine(s) addition. Addition of IL-6 and IL-12 enhanced IL-10 production in monocyte-depleted PBMC in a dose-dependent and additive manner. Furthermore, anti-IL-6 and anti-IL-12 Abs neutralized the IL-10-inductive effect of monocyte-conditioned medium. Similarly, IL-12 and IL-6 induced IL-10 production by purified CD4 + and CD8 + T cells. With respect to regulation of IL-10 produced by monocytes, TNF-α was found to induce IL-10 production by resting as well as by LPS-stimulated purified monocytes/macrophages. Taken together, these findings suggest that IL-10 production by human T cells and monocytes is differentially regulated. IL-12 and/or IL-6 can induce the expression of IL-10 by PHA-stimulated T cells, whereas TNF-α induces IL-10 production by monocytes. Since IL-10 inhibits the production of IL-6, IL-12, and TNF-α, these results may indicate a potential mechanism of negative feedback regulation of the immune response.

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