IgD-receptor up-regulation on human peripheral blood T cells in response to IgD in vitro or antigen in vivo correlates with the antibody response to influenza vaccination

Christina D. Swenson, Evan Paul Cherniack, Carlo Russo, G. Jeanette Thorbecke

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aged individuals (>65 years) were classified as antibody (Ab) responders on the basis that they showed increases to ≥1:40 in serum Ab titers to all influenza virus strains present in the trivalent influenza vaccine within 4 weeks after immunization. The peripheral blood mononuclear cells (PBMC) from pre-immunization samples of blood taken from seven Ab-responders and seven Ab-nonresponders were examined for their ability to exhibit up-regulation of IgD-receptor (IgD-R) after exposure for 2 h to immobilized cross-linked IgD, as shown by resetting with IgD-coated ox erythrocytes. The responsiveness to IgD was found to be predictive of the ability to produce Ab responses to viral protein Ag: The IgD-R up-regulation was >5 % in all Ab-responders and <4 % in all the Ab-nonresponders. In addition, there was an excellent correlation between mean Ab titers (to the three viruses in sera collected 4 weeks after immunization) and the percentage of IgD-R+ cells obtained in response to IgD in PBMC from the same individual prior to immunization: p = 0.894. Injection of influenza vaccine itself also induced IgD-R on PBMC in vivo. The percentage of IgD-R+ cells peaked after 24 h, was still detectable above background by day 7 or 14, and returned to pre-injection levels by day 28 in young subjects and aged Ab-responders, but not in Ab-nonresponders. Similarly, purified peripheral blood T cells obtained from aged Ab-responders exhibited IgD-R upon immunization in vivo. These findings suggest that Ag injection causes rapid up-regulation of IgD-R by cross-linking IgD in humans as well as in mice as shown previously. In analogy with results in mice, the present data are consistent with a role for IgD-R+ T cells in the humoral response in man. Proliferative responses to influenza proteins in peripheral blood T cells from vaccinated individuals were found to peak on day 7 and were higher in Ab-responders than in Ab-nonresponders.

Original languageEnglish
Pages (from-to)340-344
Number of pages5
JournalEuropean Journal of Immunology
Volume26
Issue number2
StatePublished - Feb 1 1996
Externally publishedYes

Fingerprint

Immunoglobulin D
Human Influenza
Antibody Formation
Blood Cells
Vaccination
Up-Regulation
T-Lymphocytes
Antigens
Antibodies
Immunization
Influenza Vaccines
In Vitro Techniques
Injections
Viral Proteins
T-Cell Antigen Receptor
Orthomyxoviridae
Serum

Keywords

  • Aging
  • IgD-receptor
  • Influenza
  • Vaccine

ASJC Scopus subject areas

  • Immunology

Cite this

IgD-receptor up-regulation on human peripheral blood T cells in response to IgD in vitro or antigen in vivo correlates with the antibody response to influenza vaccination. / Swenson, Christina D.; Cherniack, Evan Paul; Russo, Carlo; Thorbecke, G. Jeanette.

In: European Journal of Immunology, Vol. 26, No. 2, 01.02.1996, p. 340-344.

Research output: Contribution to journalArticle

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abstract = "Aged individuals (>65 years) were classified as antibody (Ab) responders on the basis that they showed increases to ≥1:40 in serum Ab titers to all influenza virus strains present in the trivalent influenza vaccine within 4 weeks after immunization. The peripheral blood mononuclear cells (PBMC) from pre-immunization samples of blood taken from seven Ab-responders and seven Ab-nonresponders were examined for their ability to exhibit up-regulation of IgD-receptor (IgD-R) after exposure for 2 h to immobilized cross-linked IgD, as shown by resetting with IgD-coated ox erythrocytes. The responsiveness to IgD was found to be predictive of the ability to produce Ab responses to viral protein Ag: The IgD-R up-regulation was >5 {\%} in all Ab-responders and <4 {\%} in all the Ab-nonresponders. In addition, there was an excellent correlation between mean Ab titers (to the three viruses in sera collected 4 weeks after immunization) and the percentage of IgD-R+ cells obtained in response to IgD in PBMC from the same individual prior to immunization: p = 0.894. Injection of influenza vaccine itself also induced IgD-R on PBMC in vivo. The percentage of IgD-R+ cells peaked after 24 h, was still detectable above background by day 7 or 14, and returned to pre-injection levels by day 28 in young subjects and aged Ab-responders, but not in Ab-nonresponders. Similarly, purified peripheral blood T cells obtained from aged Ab-responders exhibited IgD-R upon immunization in vivo. These findings suggest that Ag injection causes rapid up-regulation of IgD-R by cross-linking IgD in humans as well as in mice as shown previously. In analogy with results in mice, the present data are consistent with a role for IgD-R+ T cells in the humoral response in man. Proliferative responses to influenza proteins in peripheral blood T cells from vaccinated individuals were found to peak on day 7 and were higher in Ab-responders than in Ab-nonresponders.",
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