Idiotypes and biological activity of murine monoclonal antibodies against the hemagglutinin of measles virus

J. Gheuens, D. E. McFarlin, Kottil W Rammohan, W. J. Bellini

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Abstract

Three hybridomas, designated C2, V17, and B2, were produced from BALB/c mice after immunization with measles virus. All three were directed against the virus hemagglutinin (HA). The HA is a structural peptide of the virus and constitutes a major target for the host immune response during measles infection. The monoclonal anti-HA antibodies have biological functions such as (i) measles virus neutralization in vitro, (ii) binding to acutely and persistently infected cells, and (iii) inhibition of HA-mediated Rhesus monkey erythrocyte agglutination. Different idiotypes, designated HAMM-1, HAMM-2, and HAMM-3, were defined on C2, V17, and B2, respectively, by syngeneic anti-idiotype sera against those three monoclonal antibodies. A limited cross-reactivity with the HAMM-1 idiotype was detected in sera from some BALB/c mice immunized with measles virus. The anti-idiotype sera could significantly inhibit the biological functions of the HAMM-1 and HAMM-3 idiotypes bearing monoclonal anti-HA-antibodies. This suggests a possible role for auto-anti-idiotypes in the immune response after infection with measles virus.

Original languageEnglish
Pages (from-to)200-207
Number of pages8
JournalInfection and Immunity
Volume34
Issue number1
StatePublished - Jan 1 1981
Externally publishedYes

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Measles virus
Hemagglutinins
Monoclonal Antibodies
Anti-Idiotypic Antibodies
Serum
Viruses
Agglutination
Hybridomas
Measles
Infection
Macaca mulatta
Immunization
Erythrocytes
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Idiotypes and biological activity of murine monoclonal antibodies against the hemagglutinin of measles virus. / Gheuens, J.; McFarlin, D. E.; Rammohan, Kottil W; Bellini, W. J.

In: Infection and Immunity, Vol. 34, No. 1, 01.01.1981, p. 200-207.

Research output: Contribution to journalArticle

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