Identification of vitellogenic Atlantic blue marlin (Makaira nigricans) from muscle samples using an elisa for vitellogenin-derived yolk proteins

C. K. Tan, N. Perez, Armando J Mendez, D. Snodgrass, E. D. Prince, J. E. Serafy, F. Arocha, D. R. Schultz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The purpose of this study was to develop a direct sandwich enzyme-linked immunosorbent assay (ELISA) for vitellogenin-derived yolk proteins (VDYP) in muscle tissue of pelagic Atlantic blue marlin. Gravid ovaries were extracted with physiologic buffer, and purification steps by chromatography were monitored with a cross-reactive mouse monoclonal antibody produced to swordfish (Xiphias gladius Linnaeus, 1758) VDYP. A polyclonal goat antiserum was produced to the enriched VDYP from which specific IgG was purified by affinity chromatography, using immobilized VDYP as the immunoadsorbent. A direct sandwich ELISA was developed using the purified IgG for both capture and detection of the VDYP in standard samples up to a concentration of 1000 ng/ml, and in physiologic buffer-extracted muscle tissue of test samples, using biotin-streptavidin- peroxidase technology. The assay distinguished vitellogenic from non-vitellogenic females and males in extracts of muscle tissue from 27 specimens collected from different areas of the western North Atlantic Ocean during May-September, 2002-2003. The assay for VDYP, and a second for the fish androgen 11-ketotestosterone are in use to indicate gender of electronically-tagged blue marlin from biopsy samples taken just before fish release.

Original languageEnglish
Pages (from-to)319-329
Number of pages11
JournalBulletin of Marine Science
Volume78
Issue number2
StatePublished - Mar 1 2006

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yolk proteins
vitellogenin
muscle
muscles
protein
muscle tissues
assay
Xiphias gladius
sandwiches
sampling
chromatography
buffers
enzyme-linked immunosorbent assay
enzyme
streptavidin
androgen
goat
assays
biotin
affinity chromatography

ASJC Scopus subject areas

  • Aquatic Science
  • Oceanography

Cite this

Identification of vitellogenic Atlantic blue marlin (Makaira nigricans) from muscle samples using an elisa for vitellogenin-derived yolk proteins. / Tan, C. K.; Perez, N.; Mendez, Armando J; Snodgrass, D.; Prince, E. D.; Serafy, J. E.; Arocha, F.; Schultz, D. R.

In: Bulletin of Marine Science, Vol. 78, No. 2, 01.03.2006, p. 319-329.

Research output: Contribution to journalArticle

Tan, C. K. ; Perez, N. ; Mendez, Armando J ; Snodgrass, D. ; Prince, E. D. ; Serafy, J. E. ; Arocha, F. ; Schultz, D. R. / Identification of vitellogenic Atlantic blue marlin (Makaira nigricans) from muscle samples using an elisa for vitellogenin-derived yolk proteins. In: Bulletin of Marine Science. 2006 ; Vol. 78, No. 2. pp. 319-329.
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