Identification of vitellogenic Atlantic blue marlin (Makaira nigricans) from muscle samples using an elisa for vitellogenin-derived yolk proteins

C. K. Tan, N. Perez, A. J. Mendez, D. Snodgrass, E. D. Prince, J. E. Serafy, F. Arocha, D. R. Schultz

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2 Scopus citations

Abstract

The purpose of this study was to develop a direct sandwich enzyme-linked immunosorbent assay (ELISA) for vitellogenin-derived yolk proteins (VDYP) in muscle tissue of pelagic Atlantic blue marlin. Gravid ovaries were extracted with physiologic buffer, and purification steps by chromatography were monitored with a cross-reactive mouse monoclonal antibody produced to swordfish (Xiphias gladius Linnaeus, 1758) VDYP. A polyclonal goat antiserum was produced to the enriched VDYP from which specific IgG was purified by affinity chromatography, using immobilized VDYP as the immunoadsorbent. A direct sandwich ELISA was developed using the purified IgG for both capture and detection of the VDYP in standard samples up to a concentration of 1000 ng/ml, and in physiologic buffer-extracted muscle tissue of test samples, using biotin-streptavidin- peroxidase technology. The assay distinguished vitellogenic from non-vitellogenic females and males in extracts of muscle tissue from 27 specimens collected from different areas of the western North Atlantic Ocean during May-September, 2002-2003. The assay for VDYP, and a second for the fish androgen 11-ketotestosterone are in use to indicate gender of electronically-tagged blue marlin from biopsy samples taken just before fish release.

Original languageEnglish (US)
Pages (from-to)319-329
Number of pages11
JournalBulletin of Marine Science
Volume78
Issue number2
StatePublished - Mar 1 2006

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ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science

Cite this

Tan, C. K., Perez, N., Mendez, A. J., Snodgrass, D., Prince, E. D., Serafy, J. E., Arocha, F., & Schultz, D. R. (2006). Identification of vitellogenic Atlantic blue marlin (Makaira nigricans) from muscle samples using an elisa for vitellogenin-derived yolk proteins. Bulletin of Marine Science, 78(2), 319-329.