Identification of V3 mutations that can compensate for inactivating mutations in C4 of simian immunodeficiecy virus

F. Kirchhoff, H. G. Morrison, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A valine to isoleucine substitution at position 322 within variable region 3 (V3) of envelope of simian immunodeficiency virus was previously shown to compensate for an inactivating valine to glycine mutation at position 448 in constant region 4 (C4) (Morrison et al., Virology 195, 167-174, 1993). Cloned DNA fragments with inactivating C4 mutations were combined with complex mixtures of mutant V3 sequences, and full length genomes were transfected into COS-1 cells. By cocultivating transfected cells with CEM x 174 cells, we were able to identify two additional compensatory V3-C4 combinations. Changing 334 proline to leucine compensated for an inactivating 428 asparagine to lysine mutation and changing 324 isoleucine to leucine compensated for an inactivating 448 valine to glycine mutation. The double mutants replicated efficiently in CEM x 174 cells, rhesus monkey peripheral blood mononuclear cells, and the continuously growing rhesus monkey T cell line 221. Surprisingly, the 324 I → L and 33 P → L mutations by themselves impaired SIVmac239 wild-type replication in CEM x 174 cells. These results confirm the cooperation between V3 and C4 sequences and they define additional specific residues participating in this cooperation.

Original languageEnglish (US)
Pages (from-to)179-189
Number of pages11
JournalVirology
Volume213
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Viruses
Valine
Mutation
Isoleucine
Macaca mulatta
Leucine
Glycine
Simian Immunodeficiency Virus
Virology
Asparagine
COS Cells
Complex Mixtures
Proline
Lysine
Blood Cells
Genome
T-Lymphocytes
Cell Line
DNA

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Identification of V3 mutations that can compensate for inactivating mutations in C4 of simian immunodeficiecy virus. / Kirchhoff, F.; Morrison, H. G.; Desrosiers, Ronald Charles.

In: Virology, Vol. 213, No. 1, 1995, p. 179-189.

Research output: Contribution to journalArticle

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