Identification of TyeA residues required to interact with YopN and to regulate Yop secretion

Sabrina S. Joseph, Gregory V Plano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

14 Citations (Scopus)

Abstract

The secretion of Yops via the Yersinia type III secretion system (T3SS) is controlled, in part, by a cytoplasmic YopN/TyeA complex. This complex is required to prevent Yop secretion in the presence of extracellular calcium and prior to contact between the bacterium and a eukaryotic cell. In this study we utilized site-directed mutagenesis to analyze the role of specific TyeA regions and residues in the regulation of Yop secretion. We identified two spatially distinct, surface-exposed regions of the TyeA molecule that were required to regulate Yop secretion. One region, identified by residues M51, F55 and P56, was required for TyeA to interact with YopN. A second region, identified by residues R19, W20 and D25 was not involved in the interaction of TyeA with YopN, but may be required for the YopN/TyeA complex to interact with the T3S apparatus in a manner that blocks Yop secretion.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York
Pages235-245
Number of pages11
Volume603
ISBN (Print)9780387721231
DOIs
StatePublished - 2007

Publication series

NameAdvances in Experimental Medicine and Biology
Volume603
ISSN (Print)00652598

Fingerprint

Yersinia
Mutagenesis
Eukaryotic Cells
Site-Directed Mutagenesis
Bacteria
Calcium
Molecules
Type III Secretion Systems

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Joseph, S. S., & Plano, G. V. (2007). Identification of TyeA residues required to interact with YopN and to regulate Yop secretion. In Advances in Experimental Medicine and Biology (Vol. 603, pp. 235-245). (Advances in Experimental Medicine and Biology; Vol. 603). Springer New York. https://doi.org/10.1007/978-0-387-72124-8_21

Identification of TyeA residues required to interact with YopN and to regulate Yop secretion. / Joseph, Sabrina S.; Plano, Gregory V.

Advances in Experimental Medicine and Biology. Vol. 603 Springer New York, 2007. p. 235-245 (Advances in Experimental Medicine and Biology; Vol. 603).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Joseph, SS & Plano, GV 2007, Identification of TyeA residues required to interact with YopN and to regulate Yop secretion. in Advances in Experimental Medicine and Biology. vol. 603, Advances in Experimental Medicine and Biology, vol. 603, Springer New York, pp. 235-245. https://doi.org/10.1007/978-0-387-72124-8_21
Joseph SS, Plano GV. Identification of TyeA residues required to interact with YopN and to regulate Yop secretion. In Advances in Experimental Medicine and Biology. Vol. 603. Springer New York. 2007. p. 235-245. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-0-387-72124-8_21
Joseph, Sabrina S. ; Plano, Gregory V. / Identification of TyeA residues required to interact with YopN and to regulate Yop secretion. Advances in Experimental Medicine and Biology. Vol. 603 Springer New York, 2007. pp. 235-245 (Advances in Experimental Medicine and Biology).
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