Identification of TyeA residues required to interact with YopN and to regulate Yop secretion

Sabrina S. Joseph, Gregory V. Plano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The secretion of Yops via the Yersinia type III secretion system (T3SS) is controlled, in part, by a cytoplasmic YopN/TyeA complex. This complex is required to prevent Yop secretion in the presence of extracellular calcium and prior to contact between the bacterium and a eukaryotic cell. In this study we utilized site-directed mutagenesis to analyze the role of specific TyeA regions and residues in the regulation of Yop secretion. We identified two spatially distinct, surface-exposed regions of the TyeA molecule that were required to regulate Yop secretion. One region, identified by residues M51, F55 and P56, was required for TyeA to interact with YopN. A second region, identified by residues R19, W20 and D25 was not involved in the interaction of TyeA with YopN, but may be required for the YopN/TyeA complex to interact with the T3S apparatus in a manner that blocks Yop secretion.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
Pages235-245
Number of pages11
Volume603
DOIs
StatePublished - Dec 1 2007

Publication series

NameAdvances in Experimental Medicine and Biology
Volume603
ISSN (Print)00652598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Joseph, S. S., & Plano, G. V. (2007). Identification of TyeA residues required to interact with YopN and to regulate Yop secretion. In Advances in Experimental Medicine and Biology (Vol. 603, pp. 235-245). (Advances in Experimental Medicine and Biology; Vol. 603). https://doi.org/10.1007/978-0-387-72124-8-21