Identification of transforming genes of subgroup A and C strains of Herpesvirus saimiri

Jau U. Jung, John J. Trimble, Norval W. King, Brigitte Biesinger, Bernhard W. Fleckenstein, Ronald C. Desrosiers

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

Herpesvirus saimiri is an oncogenic herpesvirus that induces rapidly progressing lymphomas in New World primates. Using retrovirus vectors for gene transfer, specific open reading frames of H. saimiri were tested for their ability to transform rodent cells in culture. One open reading frame, designated STP-C488 (for saimiri-transformation-associated protein of the subgroup C strain 488), phenotypically transformed Rat-1 cells, resulting in formation of foci, growth at reduced serum concentration, and growth to higher cell densities. Cells transformed by STP-C488 formed invasive tumors in nude mice. The STP-A11 reading frame of strain 11 (subgroup A) was much less potent in its transforming ability than STP-C488. These results demonstrate the oncogene nature of these two open reading frames and provide a means for studying their transforming functions independent of the rest of the H. saimiri genome.

Original languageEnglish (US)
Pages (from-to)7051-7055
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number16
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Keywords

  • Collagen-like repeat
  • Focus formation
  • Tumorigenesis

ASJC Scopus subject areas

  • General

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