Identification of transforming genes of subgroup A and C strains of Herpesvirus saimiri

Jau U. Jung, John J. Trimble, Norval W. King, Brigitte Biesinger, Bernhard W. Fleckenstein, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Herpesvirus saimiri is an oncogenic herpesvirus that induces rapidly progressing lymphomas in New World primates. Using retrovirus vectors for gene transfer, specific open reading frames of H. saimiri were tested for their ability to transform rodent cells in culture. One open reading frame, designated STP-C488 (for saimiri-transformation-associated protein of the subgroup C strain 488), phenotypically transformed Rat-1 cells, resulting in formation of foci, growth at reduced serum concentration, and growth to higher cell densities. Cells transformed by STP-C488 formed invasive tumors in nude mice. The STP-A11 reading frame of strain 11 (subgroup A) was much less potent in its transforming ability than STP-C488. These results demonstrate the oncogene nature of these two open reading frames and provide a means for studying their transforming functions independent of the rest of the H. saimiri genome.

Original languageEnglish (US)
Pages (from-to)7051-7055
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number16
StatePublished - 1991
Externally publishedYes

Fingerprint

Saimiriine Herpesvirus 2
Saimiri
Protein C
Oncogenes
Open Reading Frames
Reading Frames
Herpesviridae
Retroviridae
Growth
Nude Mice
Primates
Rodentia
Lymphoma
Cell Culture Techniques
Cell Count
Genome
Serum
Genes
Neoplasms

Keywords

  • Collagen-like repeat
  • Focus formation
  • Tumorigenesis

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Identification of transforming genes of subgroup A and C strains of Herpesvirus saimiri. / Jung, Jau U.; Trimble, John J.; King, Norval W.; Biesinger, Brigitte; Fleckenstein, Bernhard W.; Desrosiers, Ronald Charles.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 16, 1991, p. 7051-7055.

Research output: Contribution to journalArticle

Jung, Jau U. ; Trimble, John J. ; King, Norval W. ; Biesinger, Brigitte ; Fleckenstein, Bernhard W. ; Desrosiers, Ronald Charles. / Identification of transforming genes of subgroup A and C strains of Herpesvirus saimiri. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 16. pp. 7051-7055.
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