Identification of the rph (RNase PH) gene of Bacillus subtilis

Evidence for suppression of cold-sensitive mutations in Escherichia coli

M. G. Craven, D. J. Henner, D. Alessi, A. T. Schauer, K. A. Ost, Murray P Deutscher, D. I. Friedman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A shotgun cloning of Bacillus subtilis DNA into pBR322 yielded a 2-kb fragment that suppresses the cold-sensitive defect of the nusA10(Cs) Escherichia coli mutant. The responsible gene encodes an open reading frame that is >50% identical at the amino acid level to the E. coli rph gene, which was formerly called orfE. This B. subtilis gene is located at 251° adjacent to the gerM gene on the B. subtilis genetic map. It has been named rph because, like its E. coli analog, it encodes a phosphate-dependent exoribonuclease activity, RNase PH, that removes the 3' nucleotides from precursor tRNAs. The cloned B. subtilis rph gene also suppresses the cold- sensitive phenotype of other unrelated cold-sensitive mutants of E. coli, but not the temperature-sensitive phenotype of three temperature-sensitive mutants, including the nusA11(Ts) mutant, that were tested.

Original languageEnglish
Pages (from-to)4727-4735
Number of pages9
JournalJournal of Bacteriology
Volume174
Issue number14
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Bacillus subtilis
Escherichia coli
Mutation
Genes
Exoribonucleases
Phenotype
Temperature
RNA Precursors
Firearms
Open Reading Frames
Organism Cloning
Nucleotides
Phosphates
ribonuclease PH
Amino Acids
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Craven, M. G., Henner, D. J., Alessi, D., Schauer, A. T., Ost, K. A., Deutscher, M. P., & Friedman, D. I. (1992). Identification of the rph (RNase PH) gene of Bacillus subtilis: Evidence for suppression of cold-sensitive mutations in Escherichia coli. Journal of Bacteriology, 174(14), 4727-4735.

Identification of the rph (RNase PH) gene of Bacillus subtilis : Evidence for suppression of cold-sensitive mutations in Escherichia coli. / Craven, M. G.; Henner, D. J.; Alessi, D.; Schauer, A. T.; Ost, K. A.; Deutscher, Murray P; Friedman, D. I.

In: Journal of Bacteriology, Vol. 174, No. 14, 01.01.1992, p. 4727-4735.

Research output: Contribution to journalArticle

Craven, MG, Henner, DJ, Alessi, D, Schauer, AT, Ost, KA, Deutscher, MP & Friedman, DI 1992, 'Identification of the rph (RNase PH) gene of Bacillus subtilis: Evidence for suppression of cold-sensitive mutations in Escherichia coli', Journal of Bacteriology, vol. 174, no. 14, pp. 4727-4735.
Craven, M. G. ; Henner, D. J. ; Alessi, D. ; Schauer, A. T. ; Ost, K. A. ; Deutscher, Murray P ; Friedman, D. I. / Identification of the rph (RNase PH) gene of Bacillus subtilis : Evidence for suppression of cold-sensitive mutations in Escherichia coli. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 14. pp. 4727-4735.
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