Identification of the rph (RNase PH) gene of Bacillus subtilis: Evidence for suppression of cold-sensitive mutations in Escherichia coli

M. G. Craven, D. J. Henner, D. Alessi, A. T. Schauer, K. A. Ost, Murray P Deutscher, D. I. Friedman

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Abstract

A shotgun cloning of Bacillus subtilis DNA into pBR322 yielded a 2-kb fragment that suppresses the cold-sensitive defect of the nusA10(Cs) Escherichia coli mutant. The responsible gene encodes an open reading frame that is >50% identical at the amino acid level to the E. coli rph gene, which was formerly called orfE. This B. subtilis gene is located at 251° adjacent to the gerM gene on the B. subtilis genetic map. It has been named rph because, like its E. coli analog, it encodes a phosphate-dependent exoribonuclease activity, RNase PH, that removes the 3' nucleotides from precursor tRNAs. The cloned B. subtilis rph gene also suppresses the cold- sensitive phenotype of other unrelated cold-sensitive mutants of E. coli, but not the temperature-sensitive phenotype of three temperature-sensitive mutants, including the nusA11(Ts) mutant, that were tested.

Original languageEnglish
Pages (from-to)4727-4735
Number of pages9
JournalJournal of Bacteriology
Volume174
Issue number14
StatePublished - Jan 1 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Craven, M. G., Henner, D. J., Alessi, D., Schauer, A. T., Ost, K. A., Deutscher, M. P., & Friedman, D. I. (1992). Identification of the rph (RNase PH) gene of Bacillus subtilis: Evidence for suppression of cold-sensitive mutations in Escherichia coli. Journal of Bacteriology, 174(14), 4727-4735.