Identification of the promoter sequences involved in the cell specific expression of the rat somatostatin gene

Ourania M. Andrisani, Timothy E. Hayes, Bernard Roos, Jack E. Dixon

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

DNA sequences containing the 5′ flanking region of the rat somatoscatin gene were linked to the coding sequence of the bacterial chloramphenicol acetyl transferase gene. This recombinant plasmid is active in expressing CAT activity in the neuronally derived, somatostatin producing CA-77 cell line. Deletion analyses of the somatostatin promoter show that the sequences proximal to position -60, relative to the cap site are required for expression of this promoter. A 4 base pair deletion of residues -46 through -43 within the somatostatin promoter results in a down mutation in vivosuggesting the existence of an element critical for the expression of the promoter in CA-77 cells. In addition, the somatostatin recombinant and its 5′ deletion constructs preferentially express CAT activity in CA-77 cells, whereas only basal level of expression is observed in HeLa, BSC4O, and RIN-5F cell lines, pointing to the cell specific nature of this promoter.

Original languageEnglish
Pages (from-to)5715-5728
Number of pages14
JournalNucleic Acids Research
Volume15
Issue number14
DOIs
StatePublished - Jul 24 1987
Externally publishedYes

Fingerprint

Somatostatin
Promoter
Rats
Genes
Cells
Gene
DNA sequences
Cell
Deletion
Cell Line
5' Flanking Region
Chloramphenicol
Transferases
Base Pairing
Line
Plasmids
DNA Sequence
Mutation
Express
Coding

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Identification of the promoter sequences involved in the cell specific expression of the rat somatostatin gene. / Andrisani, Ourania M.; Hayes, Timothy E.; Roos, Bernard; Dixon, Jack E.

In: Nucleic Acids Research, Vol. 15, No. 14, 24.07.1987, p. 5715-5728.

Research output: Contribution to journalArticle

Andrisani, Ourania M. ; Hayes, Timothy E. ; Roos, Bernard ; Dixon, Jack E. / Identification of the promoter sequences involved in the cell specific expression of the rat somatostatin gene. In: Nucleic Acids Research. 1987 ; Vol. 15, No. 14. pp. 5715-5728.
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