Identification of the major proteins that promote neuronal process outgrowth on Schwann cells in vitro

John Bixby, J. Lilien, L. F. Reichardt

Research output: Contribution to journalArticle

350 Citations (Scopus)

Abstract

Schwann cells have a unique role in regulating the growth of axons during regeneration and presumably during development. Here we show that Schwann cells are the best substrate yet identified for promoting process growth in vitro by peripheral motor neurons. To determine the molecular interactions responsible for Schwann cell regulation of axon growth, we have examined the effects of specific antibodies on process growth in vitro, and have identified three glycoproteins that play major roles. These are the Ca2+-independent cell adhesion molecule (CAM), L1/Ng-CAM; the Ca2+-dependent CAM, N-cadherin; and members of the integrin extracellular matrix receptor superfamily. Two other CAMs present on neurons and/or Schwann cells - N-CAM and myelin-associated glycoprotein - do not appear to be important in regulating process growth. Our results imply that neuronal growth cones use integrin-class extracellular matrix receptors and at least two CAMs - N-cadherin and L1/Ng-CAM - for growth on Schwann cells in vitro and establish each of these glycoproteis as a strong candidate for regulating axon growth and guidance in vivo.

Original languageEnglish
Pages (from-to)353-361
Number of pages9
JournalJournal of Cell Biology
Volume107
Issue number1
StatePublished - Jan 1 1988
Externally publishedYes

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Schwann Cells
Neural Cell Adhesion Molecule L1
Growth
Proteins
Cell Adhesion Molecules
Cadherins
Integrins
Axons
Myelin-Associated Glycoprotein
Growth Cones
Motor Neurons
In Vitro Techniques
Neuronal Outgrowth
Regeneration
Glycoproteins
Neurons
Antibodies

ASJC Scopus subject areas

  • Cell Biology

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Identification of the major proteins that promote neuronal process outgrowth on Schwann cells in vitro. / Bixby, John; Lilien, J.; Reichardt, L. F.

In: Journal of Cell Biology, Vol. 107, No. 1, 01.01.1988, p. 353-361.

Research output: Contribution to journalArticle

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