Identification of the human seminal TRH-like peptide pGlu-Phe-Pro-NH2 in normal human prostate

Peter J. Gkonos, Chuen K. Kwok, Norman L. Block, Bernard A Roos

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Human and rat prostate contain thyrotropin-releasing hormone immunoreactivity (iTRH) including TRH and an uncharged TRH-like peptide. Recently the uncharged TRH-like peptide pGlu-Phe-Pro-NH2 was purified from human semen. To determine whether this peptide was of prostatic origin, human and rat prostate extracts were analyzed by ion-exchange chromatography and reversed-phase HPLC. The predominant uncharged iTRH comigrated exactly with synthetic pGlu-Phe-Pro-NH2 on HPLC and had identical affinity to pGlu-Phe-Pro-NH2 in a TRH radioimmunoassay. We conclude that prostate is a source of this peptide in humans and rats. This amidated TRH-like peptide may play a role in human reproductive physiology.

Original languageEnglish (US)
Pages (from-to)1281-1283
Number of pages3
Issue number7
StatePublished - 1994


  • Amidated peptides
  • Neuroendocrine cells
  • Prostate
  • Thyrotropin-releasing hormone

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience


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