Identification of synaptic proteins and their isoform mRNAs in compartments of pancreatic endocrine cells

Gunilla Jacobsson, Andrew J. Bean, Richard H. Scheller, Lisa Juntti-Berggren, Jude T. Deeney, Per Olof Berggren, Björn Meister

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Several proteins that are of importance for membrane trafficking in the nerve terminal have recently been characterized. We have used Western blot and immunohistochemistry to show that synaptotagmin, synaptobrevin/VAMP (vesicle-associated membrane protein), SNAP-25 (synaptosomal-associated protein of 25 kDa), and syntaxin proteins are present in cells of the islets of Langerhans in the endocrine pancreas. Synaptotagmin-like immunoreactivity (-LI) was localized to granules within the cytoplasm of a few endocrine cells located in the periphery of the islets, identified as somatostatin-containing cells, and in many nerve fibers within the islets. VAMP-LI was seen in granules of virtually all pancreatic islet cells and also in nerve fibers. SNAP-25-LI and syntaxin-LI were predominantly present in the plasma membrane of the endocrine cells, including insulin-producing β cells. In situ hybridization, using isoform-specific oligonucleotide probes, detected VAMP- 2, cellubrevin, SNAP-25, syntaxin 1A, 4, and 5, and munc-18 mRNAs in isolated pancreatic islets and in insulin-producing cells. The results show the presence of several synaptic proteins at protein and mRNA levels in pancreatic islet cells, suggesting that they may have specific roles in the molecular regulation of exocytosis also in insulin-secreting cells.

Original languageEnglish
Pages (from-to)12487-12491
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number26
DOIs
StatePublished - Dec 20 1994
Externally publishedYes

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Endocrine Cells
Islets of Langerhans
Protein Isoforms
Qa-SNARE Proteins
Synaptosomal-Associated Protein 25
Messenger RNA
R-SNARE Proteins
Synaptotagmins
Nerve Fibers
Vesicle-Associated Membrane Protein 3
Vesicle-Associated Membrane Protein 2
Syntaxin 1
Insulin
Somatostatin-Secreting Cells
Oligonucleotide Probes
Exocytosis
Insulin-Secreting Cells
In Situ Hybridization
Membrane Proteins
Cytoplasm

Keywords

  • diabetes
  • exocytosis
  • insulin
  • secretion

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of synaptic proteins and their isoform mRNAs in compartments of pancreatic endocrine cells. / Jacobsson, Gunilla; Bean, Andrew J.; Scheller, Richard H.; Juntti-Berggren, Lisa; Deeney, Jude T.; Berggren, Per Olof; Meister, Björn.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 26, 20.12.1994, p. 12487-12491.

Research output: Contribution to journalArticle

Jacobsson, Gunilla ; Bean, Andrew J. ; Scheller, Richard H. ; Juntti-Berggren, Lisa ; Deeney, Jude T. ; Berggren, Per Olof ; Meister, Björn. / Identification of synaptic proteins and their isoform mRNAs in compartments of pancreatic endocrine cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 26. pp. 12487-12491.
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