Identification of novel steroid-response elements.

Zafar Nawaz, M. J. Tsai, D. P. McDonnell, B. W. O'Malley

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A rapid method for defining novel steroid-responsive elements has been developed. Large libraries of degenerate oligonucleotides were analyzed using a yeast-based screen to identify estrogen-responsive DNA sequences. From a library of 40,000 recombinants, seven estrogen-responsive clones were identified. When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE. One surprising result was the presence of the two half sites as direct repeats in some of the clones. This implies that in vivo estrogen receptor can bind and transactivate yeast genes through response elements in which the two half sites align as direct repeats. This protocol requires no purified protein and specifically selects for functional response elements. It has a wide application in the study of any transcription factor/DNA interaction.

Original languageEnglish
Pages (from-to)39-47
Number of pages9
JournalGene Expression
Volume2
Issue number1
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Nucleic Acid Repetitive Sequences
Response Elements
Estrogens
Clone Cells
Yeasts
Steroids
Vitellogenins
varespladib methyl
Oligonucleotides
Estrogen Receptors
Libraries
Transcription Factors
DNA
Genes
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Nawaz, Z., Tsai, M. J., McDonnell, D. P., & O'Malley, B. W. (1992). Identification of novel steroid-response elements. Gene Expression, 2(1), 39-47.

Identification of novel steroid-response elements. / Nawaz, Zafar; Tsai, M. J.; McDonnell, D. P.; O'Malley, B. W.

In: Gene Expression, Vol. 2, No. 1, 01.01.1992, p. 39-47.

Research output: Contribution to journalArticle

Nawaz, Z, Tsai, MJ, McDonnell, DP & O'Malley, BW 1992, 'Identification of novel steroid-response elements.', Gene Expression, vol. 2, no. 1, pp. 39-47.
Nawaz Z, Tsai MJ, McDonnell DP, O'Malley BW. Identification of novel steroid-response elements. Gene Expression. 1992 Jan 1;2(1):39-47.
Nawaz, Zafar ; Tsai, M. J. ; McDonnell, D. P. ; O'Malley, B. W. / Identification of novel steroid-response elements. In: Gene Expression. 1992 ; Vol. 2, No. 1. pp. 39-47.
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