Identification of miRNAs involved in DRG neurite outgrowth and their putative targets

Dario Motti, Jessica K. Lerch, Matt C. Danzi, Jared H. Gans, Frank Kuo, Tatiana I. Slepak, John Bixby, Vance Lemmon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Peripheral neurons regenerate their axons after injury. Transcriptional regulation by microRNAs (miRNAs) is one possible mechanism controlling regeneration. We profiled miRNA expression in mouse dorsal root ganglion neurons after a sciatic nerve crush, and identified 49 differentially expressed miRNAs. We evaluated the functional role of each miRNA using a phenotypic analysis approach. To predict the targets of the miRNAs we employed RNA-Sequencing and examined transcription at the isoform level. We identify thousands of differentially expressed isoforms and bioinformatically associate the miRNAs that modulate neurite growth with their putative target isoforms to outline a network of regulatory events underlying peripheral nerve regeneration. MiR-298, let-7a, and let-7f enhance neurite growth and target the majority of isoforms in the differentially expressed network.

Original languageEnglish (US)
JournalFEBS Letters
DOIs
StateAccepted/In press - 2017

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Diagnosis-Related Groups
MicroRNAs
Protein Isoforms
Neurites
Neurons
Nerve Crush
RNA Sequence Analysis
Nerve Regeneration
Spinal Ganglia
Sciatic Nerve
Transcription
Growth
Peripheral Nerves
Axons
Neuronal Outgrowth
Regeneration
RNA
Wounds and Injuries

Keywords

  • Axon growth
  • Dorsal root ganglion
  • High content analysis
  • MiRNA
  • Regeneration
  • RNA-Seq

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Identification of miRNAs involved in DRG neurite outgrowth and their putative targets. / Motti, Dario; Lerch, Jessica K.; Danzi, Matt C.; Gans, Jared H.; Kuo, Frank; Slepak, Tatiana I.; Bixby, John; Lemmon, Vance.

In: FEBS Letters, 2017.

Research output: Contribution to journalArticle

Motti, Dario ; Lerch, Jessica K. ; Danzi, Matt C. ; Gans, Jared H. ; Kuo, Frank ; Slepak, Tatiana I. ; Bixby, John ; Lemmon, Vance. / Identification of miRNAs involved in DRG neurite outgrowth and their putative targets. In: FEBS Letters. 2017.
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