Identification of hepatitis C virus by immunoelectron microscopy

X. Li, L. J. Jeffers, L. Shao, K. R. Reddy, M. de Medina, J. Scheffel, B. Moore, E. R. Schiff

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Sequencing of the hepatitis C virus (HCV) has provided a better understanding of the natural history, immunology, and epidemiology of this virus. However, the morphology of HCV has not been definitively characterized. In this study, through a sequence of concentration processes, virus-like particles were isolated from human serum and liver tissue, visualized by transmission electron microscopy and identified as hepatitis C virion by immunoelectron microscopy. Spherical flavi-like virus particles, approximately 70 nm in diameter, were observed in the fraction with 1.04-1.12 g ml-1 sucrose density and bound to immunogold particles with monoclonal antibodies (mAb) against hepatitis C. The nucleocapsid of the particles, which were 50 nm in diameter, appeared to be icosahedral in structure and surrounded by an envelope covered with surface projections. A 'tadpole' form of particles was also observed. The findings indicate that the low buoyant density in sucrose and the morphological features of the hepatitis C virion are consistent with the characteristics of flaviviruses and pestiviruses.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalJournal of viral hepatitis
Volume2
Issue number5
DOIs
StatePublished - Sep 1995

Keywords

  • buoyant density
  • hepatitis C virus
  • immunoelectron microscopy
  • morphology

ASJC Scopus subject areas

  • Hepatology
  • Infectious Diseases
  • Virology

Fingerprint Dive into the research topics of 'Identification of hepatitis C virus by immunoelectron microscopy'. Together they form a unique fingerprint.

  • Cite this

    Li, X., Jeffers, L. J., Shao, L., Reddy, K. R., de Medina, M., Scheffel, J., Moore, B., & Schiff, E. R. (1995). Identification of hepatitis C virus by immunoelectron microscopy. Journal of viral hepatitis, 2(5), 227-234. https://doi.org/10.1111/j.1365-2893.1995.tb00034.x