Identification of Gas6 as a growth factor for human Schwann cells

Rong Hao Li, Jian Chen, Glenn Hammonds, Heidi Phillips, Mark Armanini, Patrick Wood, Richard Bunge, Paul J. Godowski, Mark X. Sliwkowski, Jennie P. Mather

Research output: Contribution to journalArticle

158 Scopus citations

Abstract

Schwann cells are one of the principal components of the peripheral nervous system. They play a crucial role in nerve regeneration and can be used clinically in the repair of injured nerves. We have established serum- free, defined culture conditions that rapidly expand adult human Schwann cells without fibroblast growth. We find that Gas6, a ligand for the Axl and Rse/Tyro3 receptor protein tyrosine kinase family, stimulates human Schwann cell growth, increasing both cell number and thymidine incorporation. Gas6 has synergistic effects with the other known human Schwann cell mitogens, heregulin/glial growth factor and forskolin. Addition of Gas6 causes phosphorylation of Axl and Rse/Tyro3 simultaneously and results in ERK-2 activation. A combination of Gas6 with heregulin and forskolin, on a defined background, supports maximal Schwann cell proliferation, while preserving the typical Schwann cell morphology and expression of the Schwann cell markers S- 100, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor. Gas6 mRNA is present in both spinal motor neurons and large neurons of the dorsal root ganglia, and neural injury has been reported to upregulate Rse/Axl in the Schwann cell. This is the first demonstration of a potentially important biological role for the human Gas6/Rse-Axl system.

Original languageEnglish (US)
Pages (from-to)2012-2019
Number of pages8
JournalJournal of Neuroscience
Volume16
Issue number6
DOIs
StatePublished - Mar 15 1996

Keywords

  • Axl
  • Gas6
  • heregulin
  • human Schwann cells
  • mitogen
  • Rse
  • serum-free medium

ASJC Scopus subject areas

  • Neuroscience(all)

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    Li, R. H., Chen, J., Hammonds, G., Phillips, H., Armanini, M., Wood, P., Bunge, R., Godowski, P. J., Sliwkowski, M. X., & Mather, J. P. (1996). Identification of Gas6 as a growth factor for human Schwann cells. Journal of Neuroscience, 16(6), 2012-2019. https://doi.org/10.1523/jneurosci.16-06-02012.1996