Identification of cellular prosomatostatin and nonsomatostatin peptides derived from its amino terminus

D. C. Aron, P. C. Andrews, J. E. Dixon, B. A. Roos

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Rat prosomatostatin was isolated from a somatostatin-producing cell line and was partially microsequenced. This indicated the amino terminal structure of cellular prosomatostatin and implied a 92-amino acid sequence for the somatostatin precursor. Based on the structure for cellular prosomatostatin, a peptide was synthesized and used to develop a radioimmunoassay directed toward the amino terminal portion of prosomatostatin. This assay has revealed two peptides containing the amino-terminal portion of prosomatostatin in a somatostatin-secreting CA-77 rat medullary thyroid carcinoma cell line. These two peptides - MW 4000 and 8000 daltons - lack somatostatin immunoreactivity. Thus, processing of prosomatostatin occurs both at the amino and carboxyl regions. These results open the way for elucidation of the structure, function and metabolism of non-somatostatin peptides derived from the amino terminus of prosomatostatin.

Original languageEnglish
Pages (from-to)450-456
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume124
Issue number2
DOIs
StatePublished - Oct 30 1984
Externally publishedYes

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Peptides
Somatostatin
Cellular Structures
Rats
Cells
Cell Line
Somatostatin-Secreting Cells
prosomatostatin
Metabolism
Radioimmunoassay
Amino Acid Sequence
Assays
Amino Acids
Processing

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of cellular prosomatostatin and nonsomatostatin peptides derived from its amino terminus. / Aron, D. C.; Andrews, P. C.; Dixon, J. E.; Roos, B. A.

In: Biochemical and Biophysical Research Communications, Vol. 124, No. 2, 30.10.1984, p. 450-456.

Research output: Contribution to journalArticle

Aron, D. C. ; Andrews, P. C. ; Dixon, J. E. ; Roos, B. A. / Identification of cellular prosomatostatin and nonsomatostatin peptides derived from its amino terminus. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 124, No. 2. pp. 450-456.
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