Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli

S. Miller, L. S. Ness, C. M. Wood, B. C. Fox, I. R. Booth

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A new subunit, YabF, for the KefC K+ efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

Original languageEnglish (US)
Pages (from-to)6536-6540
Number of pages5
JournalJournal of bacteriology
Volume182
Issue number22
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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