Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli

S. Miller, L. S. Ness, C. M. Wood, B. C. Fox, I. R. Booth

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A new subunit, YabF, for the KefC K+ efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

Original languageEnglish
Pages (from-to)6536-6540
Number of pages5
JournalJournal of Bacteriology
Volume182
Issue number22
DOIs
StatePublished - Nov 15 2000

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Glutathione
Potassium
Escherichia coli
Mutation
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli. / Miller, S.; Ness, L. S.; Wood, C. M.; Fox, B. C.; Booth, I. R.

In: Journal of Bacteriology, Vol. 182, No. 22, 15.11.2000, p. 6536-6540.

Research output: Contribution to journalArticle

Miller, S. ; Ness, L. S. ; Wood, C. M. ; Fox, B. C. ; Booth, I. R. / Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli. In: Journal of Bacteriology. 2000 ; Vol. 182, No. 22. pp. 6536-6540.
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