Identification of a tetrameric hedgehog signaling complex

Melanie A. Stegman, Jefferson E. Vallance, Ganesh Elangovan, Janek Sosinski, Yan Cheng, David J. Robbins

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Hedgehog (Hh) signal transduction requires a large cytoplasmic multi-protein complex that binds microtubules in an Hh-dependent manner. Here, we show that three members of this complex, Costal2 (Cos2), Fused (Fu), and Cubitus interruptus (Ci), bind each other directly to form a trimeric complex. We demonstrate that this trimeric signaling complex exists in Drosophila lacking Suppressor of Fused (Su(fu)), an extragenic suppressor of fu, indicating that Su(fu) is not required for the formation, or apparently function, of the Hh signaling complex. However, we subsequently show that Su(fu), although not a requisite component of this complex, does form a tetrameric complex with Fu, Cos2, and Ci. This additional Su(fu)-containing Hh signaling complex does not appear to be enriched on microtubules. Additionally, we demonstrate that in response to Hh Ci accumulates in the nucleus without its various cytoplasmic binding partners, including Su(fu). We discuss a model in which Su(fu) and Cos2 each bind to Fu and Ci to exert some redundant effect on Ci such as cytoplasmic retention. This model is consistent with genetic data demonstrating that Su(fu) is not required for Hh signal transduction proper and with the elaborate genetic interactions observed among Su(fu), fu, cos2, and ci.

Original languageEnglish (US)
Pages (from-to)21809-21812
Number of pages4
JournalJournal of Biological Chemistry
Volume275
Issue number29
DOIs
StatePublished - Jul 21 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Stegman, M. A., Vallance, J. E., Elangovan, G., Sosinski, J., Cheng, Y., & Robbins, D. J. (2000). Identification of a tetrameric hedgehog signaling complex. Journal of Biological Chemistry, 275(29), 21809-21812. https://doi.org/10.1074/jbc.C000043200