Identification of a sequence element in the promoter of the Drosophila melanogaster hsp23 gene that is required for its heat activation.

R. Mestril, D. Rungger, Paul C Schiller, R. Voellmy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The expression of Drosophila melanogaster hsp23-Escherichia coli beta-galactosidase hybrid genes containing different segments of the 5' non-transcribed sequence of the hsp23 gene has been examined at the RNA and protein levels in Xenopus oocytes. Transcription of the hybrid genes is initiated correctly. Mutant genes with hsp23 gene promoter segments of at least 140 bp in length are strongly heat-activated while genes with shorter promoter segments are expressed constitutively and at low levels. This maps an element required for the heat-controlled expression of the D. melanogaster hsp23 gene to a region, approximately 140 bp upstream from the start of the transcription site, which contains a sequence (CGAGAAGTT-TCGTGT) that is closely related to the one responsible for the heat regulation of the hsp70 gene. These findings demonstrate the importance of this regulatory sequence for a second hsp gene and support the notion that hsp genes are heat-regulated by a common mechanism. The functional element in the hsp23 gene promoter is located greater than 80 bp further upstream from the TATA box than the relevant element in the hsp70 gene promoter. Even though other related sequences are present further upstream and downstream from the functional element, they play at most an auxiliary role in the regulation of hsp23 gene expression.

Original languageEnglish
Pages (from-to)2971-2976
Number of pages6
JournalEMBO Journal
Volume4
Issue number11
StatePublished - Nov 1 1985
Externally publishedYes

Fingerprint

Drosophila melanogaster
Hot Temperature
Genes
Chemical activation
TATA Box
Transcription Initiation Site
Gene Expression Regulation
Transcription
beta-Galactosidase
Xenopus
Gene expression
Escherichia coli
Oocytes
RNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Identification of a sequence element in the promoter of the Drosophila melanogaster hsp23 gene that is required for its heat activation. / Mestril, R.; Rungger, D.; Schiller, Paul C; Voellmy, R.

In: EMBO Journal, Vol. 4, No. 11, 01.11.1985, p. 2971-2976.

Research output: Contribution to journalArticle

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AU - Voellmy, R.

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AB - The expression of Drosophila melanogaster hsp23-Escherichia coli beta-galactosidase hybrid genes containing different segments of the 5' non-transcribed sequence of the hsp23 gene has been examined at the RNA and protein levels in Xenopus oocytes. Transcription of the hybrid genes is initiated correctly. Mutant genes with hsp23 gene promoter segments of at least 140 bp in length are strongly heat-activated while genes with shorter promoter segments are expressed constitutively and at low levels. This maps an element required for the heat-controlled expression of the D. melanogaster hsp23 gene to a region, approximately 140 bp upstream from the start of the transcription site, which contains a sequence (CGAGAAGTT-TCGTGT) that is closely related to the one responsible for the heat regulation of the hsp70 gene. These findings demonstrate the importance of this regulatory sequence for a second hsp gene and support the notion that hsp genes are heat-regulated by a common mechanism. The functional element in the hsp23 gene promoter is located greater than 80 bp further upstream from the TATA box than the relevant element in the hsp70 gene promoter. Even though other related sequences are present further upstream and downstream from the functional element, they play at most an auxiliary role in the regulation of hsp23 gene expression.

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