Identification of a novel M-superfamily conotoxin with the ability to enhance tetrodotoxin sensitive sodium currents

Lei Wang, Junliang Liu, Canhui Pi, Xiayun Zeng, Maojun Zhou, Xiaoyu Jiang, Shangwu Chen, Zhenghua Ren, Anlong Xu

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

In this work, a novel M-superfamily conotoxin, designated lt3a, was purified from the crude venom of Conus litteratus. Combined with peptide sequencing, MALDI-TOF mass spectrometry and cDNA cloning techniques, the amino acid sequence of lt3a was supposed to be DγCCγ OQWCDGACDCCS, where O is hydroxyproline and γ is carboxyglutamate. The Cys framework of lt3a (-CC-C-C-CC-) is similar to that of ψ-, μ-, κM-conotoxins, which are representatives of M-conotoxins. Peptide lt3a is categorized into M1 branch based on the number of residues in the last Cys loop. Whole cell patch-clamp study on adult rat dorsal root ganglion neurons indicated that lt3a could enhance tetrodotoxin-sensitive sodium currents. This is a previously unknown function of M-superfamily conotoxins.

Original languageEnglish (US)
Pages (from-to)925-932
Number of pages8
JournalArchives of Toxicology
Volume83
Issue number10
DOIs
StatePublished - Jun 29 2009
Externally publishedYes

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Keywords

  • Conus litteratus
  • M-superfamily conotoxin
  • Post-translational modification
  • Rat dorsal root ganglion neurons
  • Tetrodotoxin-sensitive sodium channels

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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