Identification of a new carcinoembryonic antigen (CEA) family member in human fetal liver - Cloning and sequence determination of pregnancy- specific glycoprotein 7

Wasif Noor Khan, Sten Hammarström

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The carcinoembryonic antigen gene family consists of the CEA- and the Pregnancy-Specific Glycoprotein- (PSG) subfamilies. Human fetal liver express several PSGs. Here we report cloning and sequencing of a new PSG subfamily member, PSG7. It is the fifth type of PSG found in fetal liver. PSG7 has the N-A1-A2-B2-C domain arrangement. Unlike other PSGs the N-terminal of PSG7 is unblocked. PSG7 has a cysteine in the C-terminal domain, which may allow dimerization. Variability analysis according to Wu and Kabat reveals that the region in the N-domain corresponding to complementarity determining region 3 of immunoglobulin is different between PSG subfamily members. Many members, including PSG7, contain the RGD sequence in this region. The CD2 region as well as two other short sequences (in N and A1 domains respectively) also show some variability. The function of PSGs is probably linked to the N-domain and the CDR2- and CD3-like regions are most likely responsible for ligand binding.

Original languageEnglish (US)
Pages (from-to)214-225
Number of pages12
JournalBiochemical and biophysical research communications
Volume168
Issue number1
DOIs
StatePublished - Apr 16 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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