Abstract
Proteins share peptidic sequences, such as a nuclear localization signal (NLS), which guide them to particular membrane-bound compartments. Similarities have also been observed within different classes of signals that target proteins to membrane-less subnuclear compartments. Common localization signals affect spatial and temporal subcellular organization and are thought to allow the coordinated response of different molecular networks to a given signaling cue. Here we identify a higher-order and predictive code, {[RR(I/L)X 3r](n, n≥1)+[L(φ/N)(V/L)](n,n >1)}, that establishes high-affinity interactions between a group of proteins and the nucleolus in response to a specific signal. This position-independent code is referred to as a nucleolar detention signal regulated by H+ (NoDSH+) and the class of proteins includes the cIAP2 apoptotic regulator, VHL ubiquitylation factor, HSC70 heat shock protein and RNF8 transcription regulator. By identifying a common subnuclear targeting consensus sequence, our work reveals rules governing the dynamics of subnuclear organization and ascribes new modes of regulation to several proteins with diverse steady-state distributions and dynamic properties.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3966-3977 |
| Number of pages | 12 |
| Journal | Molecular Biology of the Cell |
| Volume | 18 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Identification of a common subnuclear localization signal. / Mekhail, Karim; Rivero-Lopez, Luis; Al-Masri, Ahmad; Brandon, Caroline; Khacho, Mireille; Lee, Stephen.
In: Molecular Biology of the Cell, Vol. 18, No. 10, 10.2007, p. 3966-3977.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a common subnuclear localization signal
AU - Mekhail, Karim
AU - Rivero-Lopez, Luis
AU - Al-Masri, Ahmad
AU - Brandon, Caroline
AU - Khacho, Mireille
AU - Lee, Stephen
PY - 2007/10
Y1 - 2007/10
N2 - Proteins share peptidic sequences, such as a nuclear localization signal (NLS), which guide them to particular membrane-bound compartments. Similarities have also been observed within different classes of signals that target proteins to membrane-less subnuclear compartments. Common localization signals affect spatial and temporal subcellular organization and are thought to allow the coordinated response of different molecular networks to a given signaling cue. Here we identify a higher-order and predictive code, {[RR(I/L)X 3r](n, n≥1)+[L(φ/N)(V/L)](n,n >1)}, that establishes high-affinity interactions between a group of proteins and the nucleolus in response to a specific signal. This position-independent code is referred to as a nucleolar detention signal regulated by H+ (NoDSH+) and the class of proteins includes the cIAP2 apoptotic regulator, VHL ubiquitylation factor, HSC70 heat shock protein and RNF8 transcription regulator. By identifying a common subnuclear targeting consensus sequence, our work reveals rules governing the dynamics of subnuclear organization and ascribes new modes of regulation to several proteins with diverse steady-state distributions and dynamic properties.
AB - Proteins share peptidic sequences, such as a nuclear localization signal (NLS), which guide them to particular membrane-bound compartments. Similarities have also been observed within different classes of signals that target proteins to membrane-less subnuclear compartments. Common localization signals affect spatial and temporal subcellular organization and are thought to allow the coordinated response of different molecular networks to a given signaling cue. Here we identify a higher-order and predictive code, {[RR(I/L)X 3r](n, n≥1)+[L(φ/N)(V/L)](n,n >1)}, that establishes high-affinity interactions between a group of proteins and the nucleolus in response to a specific signal. This position-independent code is referred to as a nucleolar detention signal regulated by H+ (NoDSH+) and the class of proteins includes the cIAP2 apoptotic regulator, VHL ubiquitylation factor, HSC70 heat shock protein and RNF8 transcription regulator. By identifying a common subnuclear targeting consensus sequence, our work reveals rules governing the dynamics of subnuclear organization and ascribes new modes of regulation to several proteins with diverse steady-state distributions and dynamic properties.
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U2 - 10.1091/mbc.E07-03-0295
DO - 10.1091/mbc.E07-03-0295
M3 - Article
VL - 18
SP - 3966
EP - 3977
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 10
ER -