Identification of a common subnuclear localization signal

Karim Mekhail, Luis Rivero-Lopez, Ahmad Al-Masri, Caroline Brandon, Mireille Khacho, Stephen Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Proteins share peptidic sequences, such as a nuclear localization signal (NLS), which guide them to particular membrane-bound compartments. Similarities have also been observed within different classes of signals that target proteins to membrane-less subnuclear compartments. Common localization signals affect spatial and temporal subcellular organization and are thought to allow the coordinated response of different molecular networks to a given signaling cue. Here we identify a higher-order and predictive code, {[RR(I/L)X 3r](n, n≥1)+[L(φ/N)(V/L)](n,n >1)}, that establishes high-affinity interactions between a group of proteins and the nucleolus in response to a specific signal. This position-independent code is referred to as a nucleolar detention signal regulated by H+ (NoDSH+) and the class of proteins includes the cIAP2 apoptotic regulator, VHL ubiquitylation factor, HSC70 heat shock protein and RNF8 transcription regulator. By identifying a common subnuclear targeting consensus sequence, our work reveals rules governing the dynamics of subnuclear organization and ascribes new modes of regulation to several proteins with diverse steady-state distributions and dynamic properties.

Original languageEnglish (US)
Pages (from-to)3966-3977
Number of pages12
JournalMolecular Biology of the Cell
Volume18
Issue number10
DOIs
StatePublished - Oct 2007
Externally publishedYes

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Proteins
HSC70 Heat-Shock Proteins
Nuclear Localization Signals
Ubiquitination
Consensus Sequence
Cues
Membrane Proteins
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Mekhail, K., Rivero-Lopez, L., Al-Masri, A., Brandon, C., Khacho, M., & Lee, S. (2007). Identification of a common subnuclear localization signal. Molecular Biology of the Cell, 18(10), 3966-3977. https://doi.org/10.1091/mbc.E07-03-0295

Identification of a common subnuclear localization signal. / Mekhail, Karim; Rivero-Lopez, Luis; Al-Masri, Ahmad; Brandon, Caroline; Khacho, Mireille; Lee, Stephen.

In: Molecular Biology of the Cell, Vol. 18, No. 10, 10.2007, p. 3966-3977.

Research output: Contribution to journalArticle

Mekhail, K, Rivero-Lopez, L, Al-Masri, A, Brandon, C, Khacho, M & Lee, S 2007, 'Identification of a common subnuclear localization signal', Molecular Biology of the Cell, vol. 18, no. 10, pp. 3966-3977. https://doi.org/10.1091/mbc.E07-03-0295
Mekhail K, Rivero-Lopez L, Al-Masri A, Brandon C, Khacho M, Lee S. Identification of a common subnuclear localization signal. Molecular Biology of the Cell. 2007 Oct;18(10):3966-3977. https://doi.org/10.1091/mbc.E07-03-0295
Mekhail, Karim ; Rivero-Lopez, Luis ; Al-Masri, Ahmad ; Brandon, Caroline ; Khacho, Mireille ; Lee, Stephen. / Identification of a common subnuclear localization signal. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 10. pp. 3966-3977.
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