Identification of a CD11b+/Gr-1+/CD31+ myeloid progenitor capable of activating or suppressing CD8+ T cells

V. Bronte, E. Apolloni, A. Cabrelle, R. Ronca, Paolo Serafini, P. Zamboni, N. P. Restifo, P. Zanovello

Research output: Contribution to journalArticle

412 Citations (Scopus)

Abstract

Apoptotic death of CD8+ T cells can be induced by a population of inhibitory myeloid cells that are double positive for the CD11b and Gr-1 markers. These cells are responsible for the immunosuppression observed in pathologies as dissimilar as tumor growth and overwhelming infections, or after immunization with viruses. The appearance of a CD11b+/Gr-1+ population of inhibitory macrophages (iMacs) could be attributed to high levels of granulocyte-macrophage colony-stimulating factor (GM-CSF) in vivo. Deletion of iMacs in vitro or in vivo reversed the depression of CD8+ T-cell function. We isolated iMacs from the spleens of immunocompromised mice and found that these cells were positive for CD31, ER-MP20 (Ly-6C), and ER-MP58, markers characteristic of granulocyte/monocyte precursors. Importantly, although iMacs retained their inhibitory properties when cultured in vitro in standard medium, suppressive functions could be modulated by cytokine exposure. Whereas culture with the cytokine interleukin 4 (IL-4) increased iMac inhibitory activity, these cells could be differentiated into a nonadherent population of fully mature and highly activated dendritic cells when cultured in the presence of IL-4 and GM-CSF. A common CD31+/CD11b+/Gr-1+ progenitor can thus give rise to cells capable of either activating or inhibiting the function of CD8+ T lymphocytes, depending on the cytokine milieu that prevails during antigen-presenting cell maturation. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)3838-3846
Number of pages9
JournalBlood
Volume96
Issue number12
StatePublished - Dec 1 2000
Externally publishedYes

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T-cells
Macrophages
T-Lymphocytes
Granulocyte-Macrophage Colony-Stimulating Factor
Cytokines
Interleukin-4
Population
Immunization
Pathology
Antigen-Presenting Cells
Myeloid Cells
Viruses
Granulocytes
Immunosuppression
Dendritic Cells
Tumors
Monocytes
Spleen
Cells
Growth

ASJC Scopus subject areas

  • Hematology

Cite this

Bronte, V., Apolloni, E., Cabrelle, A., Ronca, R., Serafini, P., Zamboni, P., ... Zanovello, P. (2000). Identification of a CD11b+/Gr-1+/CD31+ myeloid progenitor capable of activating or suppressing CD8+ T cells. Blood, 96(12), 3838-3846.

Identification of a CD11b+/Gr-1+/CD31+ myeloid progenitor capable of activating or suppressing CD8+ T cells. / Bronte, V.; Apolloni, E.; Cabrelle, A.; Ronca, R.; Serafini, Paolo; Zamboni, P.; Restifo, N. P.; Zanovello, P.

In: Blood, Vol. 96, No. 12, 01.12.2000, p. 3838-3846.

Research output: Contribution to journalArticle

Bronte, V, Apolloni, E, Cabrelle, A, Ronca, R, Serafini, P, Zamboni, P, Restifo, NP & Zanovello, P 2000, 'Identification of a CD11b+/Gr-1+/CD31+ myeloid progenitor capable of activating or suppressing CD8+ T cells', Blood, vol. 96, no. 12, pp. 3838-3846.
Bronte V, Apolloni E, Cabrelle A, Ronca R, Serafini P, Zamboni P et al. Identification of a CD11b+/Gr-1+/CD31+ myeloid progenitor capable of activating or suppressing CD8+ T cells. Blood. 2000 Dec 1;96(12):3838-3846.
Bronte, V. ; Apolloni, E. ; Cabrelle, A. ; Ronca, R. ; Serafini, Paolo ; Zamboni, P. ; Restifo, N. P. ; Zanovello, P. / Identification of a CD11b+/Gr-1+/CD31+ myeloid progenitor capable of activating or suppressing CD8+ T cells. In: Blood. 2000 ; Vol. 96, No. 12. pp. 3838-3846.
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AU - Serafini, Paolo

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