TY - JOUR
T1 - Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells
AU - Morrish, D. W.
AU - Linetsky, E.
AU - Bhardwaj, D.
AU - Li, H.
AU - Dakour, J.
AU - Marsh, R. G.
AU - Paterson, M. C.
AU - Godbout, R.
PY - 1996/9
Y1 - 1996/9
N2 - We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.
AB - We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.
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U2 - 10.1016/S0143-4004(96)90025-9
DO - 10.1016/S0143-4004(96)90025-9
M3 - Article
C2 - 8899872
AN - SCOPUS:0029845298
VL - 17
SP - 431
EP - 441
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 7
ER -