Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells

D. W. Morrish, Elina Linetsky, D. Bhardwaj, H. Li, J. Dakour, R. G. Marsh, M. C. Paterson, R. Godbout

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.

Original languageEnglish (US)
Pages (from-to)431-441
Number of pages11
JournalPlacenta
Volume17
Issue number7
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Trophoblasts
Genes
Epidermal Growth Factor
Placenta
3-Hydroxysteroid Dehydrogenases
Mitochondrial Proton-Translocating ATPases
HSP27 Heat-Shock Proteins
Keratin-8
Keratin-19
Adrenomedullin
Messenger RNA
Macrophage Colony-Stimulating Factor
Plasminogen Activator Inhibitor 1
Transforming Growth Factors
Granulocyte Colony-Stimulating Factor
Chorionic Gonadotropin
Gene Library
Fibronectins
Superoxide Dismutase
Zinc

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells. / Morrish, D. W.; Linetsky, Elina; Bhardwaj, D.; Li, H.; Dakour, J.; Marsh, R. G.; Paterson, M. C.; Godbout, R.

In: Placenta, Vol. 17, No. 7, 01.01.1996, p. 431-441.

Research output: Contribution to journalArticle

Morrish, D. W. ; Linetsky, Elina ; Bhardwaj, D. ; Li, H. ; Dakour, J. ; Marsh, R. G. ; Paterson, M. C. ; Godbout, R. / Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells. In: Placenta. 1996 ; Vol. 17, No. 7. pp. 431-441.
@article{6775d3794cc24f4f960051d740600c5d,
title = "Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells",
abstract = "We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.",
author = "Morrish, {D. W.} and Elina Linetsky and D. Bhardwaj and H. Li and J. Dakour and Marsh, {R. G.} and Paterson, {M. C.} and R. Godbout",
year = "1996",
month = "1",
day = "1",
doi = "10.1016/S0143-4004(96)90025-9",
language = "English (US)",
volume = "17",
pages = "431--441",
journal = "Placenta",
issn = "0143-4004",
publisher = "W.B. Saunders Ltd",
number = "7",

}

TY - JOUR

T1 - Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells

AU - Morrish, D. W.

AU - Linetsky, Elina

AU - Bhardwaj, D.

AU - Li, H.

AU - Dakour, J.

AU - Marsh, R. G.

AU - Paterson, M. C.

AU - Godbout, R.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.

AB - We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0029845298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029845298&partnerID=8YFLogxK

U2 - 10.1016/S0143-4004(96)90025-9

DO - 10.1016/S0143-4004(96)90025-9

M3 - Article

VL - 17

SP - 431

EP - 441

JO - Placenta

JF - Placenta

SN - 0143-4004

IS - 7

ER -