Abstract
We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 431-441 |
| Number of pages | 11 |
| Journal | Placenta |
| Volume | 17 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jan 1 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology
- Developmental Biology
Cite this
Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells. / Morrish, D. W.; Linetsky, Elina; Bhardwaj, D.; Li, H.; Dakour, J.; Marsh, R. G.; Paterson, M. C.; Godbout, R.
In: Placenta, Vol. 17, No. 7, 01.01.1996, p. 431-441.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells
AU - Morrish, D. W.
AU - Linetsky, Elina
AU - Bhardwaj, D.
AU - Li, H.
AU - Dakour, J.
AU - Marsh, R. G.
AU - Paterson, M. C.
AU - Godbout, R.
PY - 1996/1/1
Y1 - 1996/1/1
N2 - We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.
AB - We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocyte-monocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation, we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin 8, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0029845298&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029845298&partnerID=8YFLogxK
U2 - 10.1016/S0143-4004(96)90025-9
DO - 10.1016/S0143-4004(96)90025-9
M3 - Article
C2 - 8899872
AN - SCOPUS:0029845298
VL - 17
SP - 431
EP - 441
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 7
ER -