Identification and measurement of β-endorphin levels in the skin during induced hair growth in mice

Jens Furkert, Ulrich Klug, Andrzej Slominski, Stefan Eichmüller, Burkhard Mehlis, Uwe Kertscher, Ralf Paus

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We describe new and effective techniques for extracting proopiomelanocortin (POMC)-derived peptides from mammalian skin. Using this methodology (hot-acid extraction) and two independent HPLC-controlled RIA systems, we identify β-endorphin peptide in mammalian skin and demonstrate significant hair cycle-dependent fluctuations in both the skin concentration and the in situ expression pattern of β-endorphin (sebaceous glands) during the entire murine hair cycle. The observed anagen (growth phase)-associated increase in β-endorphin concentration and its decline during the follicle involution (catagen) or resting (telogen) phase raise the possibility of a regulatory function of this neuropeptide in cyclic changes of skin physiology.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume1336
Issue number2
DOIs
StatePublished - Aug 27 1997
Externally publishedYes

Fingerprint

Endorphins
Hair
Skin
Skin Physiological Phenomena
Growth
Sebaceous Glands
Pro-Opiomelanocortin
Peptides
Neuropeptides
Physiology
High Pressure Liquid Chromatography
Acids

Keywords

  • β-Endorphin
  • C57BL)/6 mouse
  • Hair cycle
  • Neuropeptide isolation
  • Proopiomelanocortin
  • Sebaceous gland

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Identification and measurement of β-endorphin levels in the skin during induced hair growth in mice. / Furkert, Jens; Klug, Ulrich; Slominski, Andrzej; Eichmüller, Stefan; Mehlis, Burkhard; Kertscher, Uwe; Paus, Ralf.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1336, No. 2, 27.08.1997, p. 315-322.

Research output: Contribution to journalArticle

Furkert, Jens ; Klug, Ulrich ; Slominski, Andrzej ; Eichmüller, Stefan ; Mehlis, Burkhard ; Kertscher, Uwe ; Paus, Ralf. / Identification and measurement of β-endorphin levels in the skin during induced hair growth in mice. In: Biochimica et Biophysica Acta - General Subjects. 1997 ; Vol. 1336, No. 2. pp. 315-322.
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AU - Kertscher, Uwe

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