Identification and measurement of β-endorphin levels in the skin during induced hair growth in mice

Jens Furkert, Ulrich Klug, Andrzej Slominski, Stefan Eichmüller, Burkhard Mehlis, Uwe Kertscher, Ralf Paus

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We describe new and effective techniques for extracting proopiomelanocortin (POMC)-derived peptides from mammalian skin. Using this methodology (hot-acid extraction) and two independent HPLC-controlled RIA systems, we identify β-endorphin peptide in mammalian skin and demonstrate significant hair cycle-dependent fluctuations in both the skin concentration and the in situ expression pattern of β-endorphin (sebaceous glands) during the entire murine hair cycle. The observed anagen (growth phase)-associated increase in β-endorphin concentration and its decline during the follicle involution (catagen) or resting (telogen) phase raise the possibility of a regulatory function of this neuropeptide in cyclic changes of skin physiology.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume1336
Issue number2
DOIs
StatePublished - Aug 27 1997
Externally publishedYes

Keywords

  • C57BL)/6 mouse
  • Hair cycle
  • Neuropeptide isolation
  • Proopiomelanocortin
  • Sebaceous gland
  • β-Endorphin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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