Identification and localization of an enhancer for the human λ L chain Ig gene complex

Bonnie B Blomberg, Charles M. Rudin, Ursula Storb

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A strong transcriptional enhancer was identified for the human λ L chain Ig gene complex. Enhancer activity was measured by activation of the chloramphenicol acetyl transferase (CAT) gene in a transient assay using both mouse and human B lymphoid cell lines. The smallest fragment identified with enhancer activity was 111 bp, which resides 11.7 kb downstream (3′) of Cλ7, a constant region gene we have recently isolated and identified as functional in the human population. Enhancer activity is orientation independent, tissue specific (active in all B cell lines tested and not in a T cell line), and independent of NFκB, similar to the mouse λ enhancers recently reported. The human λ enhancer is active in both mouse and human B cell lines; interestingly, the mouse λ enhancers are active in mouse lines but not in a human B cell line. DNA sequence comparison of the mouse and human λ enhancers indicates a higher degree of homology (average of 72.5%) within the 111-bp enhancer core region identified here than for the remaining flanking sequence compared (average of 42%). This discovery of an enhancer in the human λ locus (HuEλ), which is clearly distinct from that of the H and L chain loci, will help to determine the mechanism for the ordered expression and rearrangement of these gene complexes in B cell ontogeny. The presence of only one enhancer in the human Cλ complex 3′ of all the C genes suggests that the evolutionary duplication of the L locus differs from that seen in mouse; in mouse the duplication unit was JCJC-enhancer, whereas the human JCλs duplicated without the enhancer.

Original languageEnglish
Pages (from-to)2354-2358
Number of pages5
JournalJournal of Immunology
Volume147
Issue number7
StatePublished - Oct 1 1991

Fingerprint

Immunoglobulin Genes
Cell Line
B-Lymphocytes
Genes
Gene Rearrangement
Chloramphenicol
Transferases
Lymphocytes
T-Lymphocytes
Gene Expression

ASJC Scopus subject areas

  • Immunology

Cite this

Identification and localization of an enhancer for the human λ L chain Ig gene complex. / Blomberg, Bonnie B; Rudin, Charles M.; Storb, Ursula.

In: Journal of Immunology, Vol. 147, No. 7, 01.10.1991, p. 2354-2358.

Research output: Contribution to journalArticle

Blomberg, Bonnie B ; Rudin, Charles M. ; Storb, Ursula. / Identification and localization of an enhancer for the human λ L chain Ig gene complex. In: Journal of Immunology. 1991 ; Vol. 147, No. 7. pp. 2354-2358.
@article{00c31a08ce014a78b4c631dd1201c5e1,
title = "Identification and localization of an enhancer for the human λ L chain Ig gene complex",
abstract = "A strong transcriptional enhancer was identified for the human λ L chain Ig gene complex. Enhancer activity was measured by activation of the chloramphenicol acetyl transferase (CAT) gene in a transient assay using both mouse and human B lymphoid cell lines. The smallest fragment identified with enhancer activity was 111 bp, which resides 11.7 kb downstream (3′) of Cλ7, a constant region gene we have recently isolated and identified as functional in the human population. Enhancer activity is orientation independent, tissue specific (active in all B cell lines tested and not in a T cell line), and independent of NFκB, similar to the mouse λ enhancers recently reported. The human λ enhancer is active in both mouse and human B cell lines; interestingly, the mouse λ enhancers are active in mouse lines but not in a human B cell line. DNA sequence comparison of the mouse and human λ enhancers indicates a higher degree of homology (average of 72.5{\%}) within the 111-bp enhancer core region identified here than for the remaining flanking sequence compared (average of 42{\%}). This discovery of an enhancer in the human λ locus (HuEλ), which is clearly distinct from that of the H and L chain loci, will help to determine the mechanism for the ordered expression and rearrangement of these gene complexes in B cell ontogeny. The presence of only one enhancer in the human Cλ complex 3′ of all the C genes suggests that the evolutionary duplication of the L locus differs from that seen in mouse; in mouse the duplication unit was JCJC-enhancer, whereas the human JCλs duplicated without the enhancer.",
author = "Blomberg, {Bonnie B} and Rudin, {Charles M.} and Ursula Storb",
year = "1991",
month = "10",
day = "1",
language = "English",
volume = "147",
pages = "2354--2358",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

TY - JOUR

T1 - Identification and localization of an enhancer for the human λ L chain Ig gene complex

AU - Blomberg, Bonnie B

AU - Rudin, Charles M.

AU - Storb, Ursula

PY - 1991/10/1

Y1 - 1991/10/1

N2 - A strong transcriptional enhancer was identified for the human λ L chain Ig gene complex. Enhancer activity was measured by activation of the chloramphenicol acetyl transferase (CAT) gene in a transient assay using both mouse and human B lymphoid cell lines. The smallest fragment identified with enhancer activity was 111 bp, which resides 11.7 kb downstream (3′) of Cλ7, a constant region gene we have recently isolated and identified as functional in the human population. Enhancer activity is orientation independent, tissue specific (active in all B cell lines tested and not in a T cell line), and independent of NFκB, similar to the mouse λ enhancers recently reported. The human λ enhancer is active in both mouse and human B cell lines; interestingly, the mouse λ enhancers are active in mouse lines but not in a human B cell line. DNA sequence comparison of the mouse and human λ enhancers indicates a higher degree of homology (average of 72.5%) within the 111-bp enhancer core region identified here than for the remaining flanking sequence compared (average of 42%). This discovery of an enhancer in the human λ locus (HuEλ), which is clearly distinct from that of the H and L chain loci, will help to determine the mechanism for the ordered expression and rearrangement of these gene complexes in B cell ontogeny. The presence of only one enhancer in the human Cλ complex 3′ of all the C genes suggests that the evolutionary duplication of the L locus differs from that seen in mouse; in mouse the duplication unit was JCJC-enhancer, whereas the human JCλs duplicated without the enhancer.

AB - A strong transcriptional enhancer was identified for the human λ L chain Ig gene complex. Enhancer activity was measured by activation of the chloramphenicol acetyl transferase (CAT) gene in a transient assay using both mouse and human B lymphoid cell lines. The smallest fragment identified with enhancer activity was 111 bp, which resides 11.7 kb downstream (3′) of Cλ7, a constant region gene we have recently isolated and identified as functional in the human population. Enhancer activity is orientation independent, tissue specific (active in all B cell lines tested and not in a T cell line), and independent of NFκB, similar to the mouse λ enhancers recently reported. The human λ enhancer is active in both mouse and human B cell lines; interestingly, the mouse λ enhancers are active in mouse lines but not in a human B cell line. DNA sequence comparison of the mouse and human λ enhancers indicates a higher degree of homology (average of 72.5%) within the 111-bp enhancer core region identified here than for the remaining flanking sequence compared (average of 42%). This discovery of an enhancer in the human λ locus (HuEλ), which is clearly distinct from that of the H and L chain loci, will help to determine the mechanism for the ordered expression and rearrangement of these gene complexes in B cell ontogeny. The presence of only one enhancer in the human Cλ complex 3′ of all the C genes suggests that the evolutionary duplication of the L locus differs from that seen in mouse; in mouse the duplication unit was JCJC-enhancer, whereas the human JCλs duplicated without the enhancer.

UR - http://www.scopus.com/inward/record.url?scp=0025988320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025988320&partnerID=8YFLogxK

M3 - Article

C2 - 1918967

AN - SCOPUS:0025988320

VL - 147

SP - 2354

EP - 2358

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 7

ER -