Identification and characterization of two isozymic forms of arylamine n-acetyltransferase in syrian hamster skin

Stephen J. Gaudet, Andrzej Slominski, Mohammad Etminan, Daniel Pruski, Ralf Paus, M. A.A. Namboodiri

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Arylamine N-acetyltransferase (EC 2.3.1.5) activity was examined using skin from Syrian hamster. Two isozymes of arylamine N-acetyltransferase, designated NAT-1 and NAT-2, were detected on anion-exchange high-performance liquid chromatography analysis. Both enzyme activities had indistinguishable molecular masses (30 kDa), but differed significantly in their specificity toward the aromatic amines including serotonin, dopamine, methoxytryptamine, tryptamine, para-phenetidine, para-aminobenzoic acid, and sulphamethazine. Specifically, NAT-2 but not NAT-1 catalyzed acetylation of dopamine to N-acetyldopamine and acetylation of serotonin to form N-acetylserotonin, a direct precursor of melatonin. The two isozymes were also distinguishable based upon their sensitivity toward methotrexate inhibition (50% inhibiting dose for NAT-1 = 380 μM; NAT-2 > 2 mM). The presence of these two activities in the skin raises new questions about the physiologic role of this enzyme in general and in the skin-specific functions in particular.

Original languageEnglish (US)
Pages (from-to)660-665
Number of pages6
JournalJournal of Investigative Dermatology
Volume101
Issue number5
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Arylamine N-Acetyltransferase
Acetyltransferases
Mesocricetus
Skin
Acetylation
Isoenzymes
Phenetidine
Dopamine
Serotonin
5-Methoxytryptamine
4-Aminobenzoic Acid
High performance liquid chromatography
Enzyme activity
Molecular mass
Melatonin
Enzymes
Methotrexate
Amines
Anions
N-acetyltalosaminuronic acid

Keywords

  • detoxification
  • melatonin
  • N-acetyldopamine
  • N-acetylserotonin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Identification and characterization of two isozymic forms of arylamine n-acetyltransferase in syrian hamster skin. / Gaudet, Stephen J.; Slominski, Andrzej; Etminan, Mohammad; Pruski, Daniel; Paus, Ralf; Namboodiri, M. A.A.

In: Journal of Investigative Dermatology, Vol. 101, No. 5, 01.01.1993, p. 660-665.

Research output: Contribution to journalArticle

Gaudet, Stephen J. ; Slominski, Andrzej ; Etminan, Mohammad ; Pruski, Daniel ; Paus, Ralf ; Namboodiri, M. A.A. / Identification and characterization of two isozymic forms of arylamine n-acetyltransferase in syrian hamster skin. In: Journal of Investigative Dermatology. 1993 ; Vol. 101, No. 5. pp. 660-665.
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