Abstract
Arylamine N-acetyltransferase (EC 2.3.1.5) activity was examined using skin from Syrian hamster. Two isozymes of arylamine N-acetyltransferase, designated NAT-1 and NAT-2, were detected on anion-exchange high-performance liquid chromatography analysis. Both enzyme activities had indistinguishable molecular masses (30 kDa), but differed significantly in their specificity toward the aromatic amines including serotonin, dopamine, methoxytryptamine, tryptamine, para-phenetidine, para-aminobenzoic acid, and sulphamethazine. Specifically, NAT-2 but not NAT-1 catalyzed acetylation of dopamine to N-acetyldopamine and acetylation of serotonin to form N-acetylserotonin, a direct precursor of melatonin. The two isozymes were also distinguishable based upon their sensitivity toward methotrexate inhibition (50% inhibiting dose for NAT-1 = 380 μM; NAT-2 > 2 mM). The presence of these two activities in the skin raises new questions about the physiologic role of this enzyme in general and in the skin-specific functions in particular.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 660-665 |
| Number of pages | 6 |
| Journal | Journal of Investigative Dermatology |
| Volume | 101 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 1993 |
| Externally published | Yes |
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Keywords
- detoxification
- melatonin
- N-acetyldopamine
- N-acetylserotonin
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Dermatology
- Cell Biology
Cite this
Identification and characterization of two isozymic forms of arylamine n-acetyltransferase in syrian hamster skin. / Gaudet, Stephen J.; Slominski, Andrzej; Etminan, Mohammad; Pruski, Daniel; Paus, Ralf; Namboodiri, M. A.A.
In: Journal of Investigative Dermatology, Vol. 101, No. 5, 01.01.1993, p. 660-665.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification and characterization of two isozymic forms of arylamine n-acetyltransferase in syrian hamster skin
AU - Gaudet, Stephen J.
AU - Slominski, Andrzej
AU - Etminan, Mohammad
AU - Pruski, Daniel
AU - Paus, Ralf
AU - Namboodiri, M. A.A.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Arylamine N-acetyltransferase (EC 2.3.1.5) activity was examined using skin from Syrian hamster. Two isozymes of arylamine N-acetyltransferase, designated NAT-1 and NAT-2, were detected on anion-exchange high-performance liquid chromatography analysis. Both enzyme activities had indistinguishable molecular masses (30 kDa), but differed significantly in their specificity toward the aromatic amines including serotonin, dopamine, methoxytryptamine, tryptamine, para-phenetidine, para-aminobenzoic acid, and sulphamethazine. Specifically, NAT-2 but not NAT-1 catalyzed acetylation of dopamine to N-acetyldopamine and acetylation of serotonin to form N-acetylserotonin, a direct precursor of melatonin. The two isozymes were also distinguishable based upon their sensitivity toward methotrexate inhibition (50% inhibiting dose for NAT-1 = 380 μM; NAT-2 > 2 mM). The presence of these two activities in the skin raises new questions about the physiologic role of this enzyme in general and in the skin-specific functions in particular.
AB - Arylamine N-acetyltransferase (EC 2.3.1.5) activity was examined using skin from Syrian hamster. Two isozymes of arylamine N-acetyltransferase, designated NAT-1 and NAT-2, were detected on anion-exchange high-performance liquid chromatography analysis. Both enzyme activities had indistinguishable molecular masses (30 kDa), but differed significantly in their specificity toward the aromatic amines including serotonin, dopamine, methoxytryptamine, tryptamine, para-phenetidine, para-aminobenzoic acid, and sulphamethazine. Specifically, NAT-2 but not NAT-1 catalyzed acetylation of dopamine to N-acetyldopamine and acetylation of serotonin to form N-acetylserotonin, a direct precursor of melatonin. The two isozymes were also distinguishable based upon their sensitivity toward methotrexate inhibition (50% inhibiting dose for NAT-1 = 380 μM; NAT-2 > 2 mM). The presence of these two activities in the skin raises new questions about the physiologic role of this enzyme in general and in the skin-specific functions in particular.
KW - detoxification
KW - melatonin
KW - N-acetyldopamine
KW - N-acetylserotonin
UR - http://www.scopus.com/inward/record.url?scp=0027527685&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027527685&partnerID=8YFLogxK
U2 - 10.1111/1523-1747.ep12371672
DO - 10.1111/1523-1747.ep12371672
M3 - Article
C2 - 8228325
AN - SCOPUS:0027527685
VL - 101
SP - 660
EP - 665
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 5
ER -