Identification and characterization of the Escherichia coli rbn gene encoding the tRNA processing enzyme RNase BN

Colleen Callahan, Murray P Deutscher

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Abstract

The gene encoding RNase BN was localized to 88 min on the Escherichia coli chromosome by a novel suppressor assay and conjugational and transductional analysis. Assay of subclones derived from lambda phage 543 of the Kohara library, which encompasses this region of the chromosome, for elevated RNase BN activity identified o290, a previously reported open reading frame, as the gene encoding RNase BN. Interruption of this gene with a Kan(r) cassette and introduction into the chromosome eliminated cellular RNase BN activity but had no effect on cell growth. On the basis of these data, we suggest that o290 be renamed rbn. Potential homologs of rbn in other organisms also were identified.

Original languageEnglish
Pages (from-to)7329-7332
Number of pages4
JournalJournal of Bacteriology
Volume178
Issue number24
StatePublished - Dec 1 1996

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ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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