Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil

Artur Trancoso Lopo de Queiroz, Luciane Amorim Santos, Domingos Ramon Moreau, Tulio de Oliveira, David Watkins, Bernardo Galvão-Castro, Luiz Carlos Junior Alcantara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Genetic analysis of HIV-1 is essential to improve treatment strategies and select epitopes for vaccine programs. The objective of this study was to determine whether known CD4+ and CD8+ epitopes were present in Brazilian HIV-1 strains. We used previously described CD8+ and CD4+ epitopes from the Los Alamos laboratory to search for these epitopes in the Brazilian sequences using the HIVbase program and we compared the frequency results with the analyses using physical-chemical profile tools from Network Protein Sequence Analysis (NPSA), and the SYFPEITHI program. Furthermore, this analysis was carried out with the Prosite tool using the GeneDoc program and ds/dn analyses using the Synonymous Nonsynonymous Analysis Program (SNAP). The HIVbase epitope mapping demonstrated that 30 CD8+ and 6 CD4+ epitopes were present in the Brazilian sequences at a high frequency. Only two of these epitopes were heavily glycosylated. Interestingly, ds/dn analyses showed evidence of purifying selective pressure. These types of analyses could be useful for the assessment of possible vaccine efficiency in populations.

Original languageEnglish
Pages (from-to)27-30
Number of pages4
JournalBrazilian Journal of Infectious Diseases
Volume11
Issue number1
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

Brazil
HIV-1
Epitopes
Vaccines
Epitope Mapping
Protein Sequence Analysis
Population

Keywords

  • Env
  • Epitope
  • HIV-1
  • Vaccine

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Lopo de Queiroz, A. T., Santos, L. A., Moreau, D. R., de Oliveira, T., Watkins, D., Galvão-Castro, B., & Alcantara, L. C. J. (2007). Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil. Brazilian Journal of Infectious Diseases, 11(1), 27-30.

Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil. / Lopo de Queiroz, Artur Trancoso; Santos, Luciane Amorim; Moreau, Domingos Ramon; de Oliveira, Tulio; Watkins, David; Galvão-Castro, Bernardo; Alcantara, Luiz Carlos Junior.

In: Brazilian Journal of Infectious Diseases, Vol. 11, No. 1, 01.02.2007, p. 27-30.

Research output: Contribution to journalArticle

Lopo de Queiroz, AT, Santos, LA, Moreau, DR, de Oliveira, T, Watkins, D, Galvão-Castro, B & Alcantara, LCJ 2007, 'Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil', Brazilian Journal of Infectious Diseases, vol. 11, no. 1, pp. 27-30.
Lopo de Queiroz AT, Santos LA, Moreau DR, de Oliveira T, Watkins D, Galvão-Castro B et al. Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil. Brazilian Journal of Infectious Diseases. 2007 Feb 1;11(1):27-30.
Lopo de Queiroz, Artur Trancoso ; Santos, Luciane Amorim ; Moreau, Domingos Ramon ; de Oliveira, Tulio ; Watkins, David ; Galvão-Castro, Bernardo ; Alcantara, Luiz Carlos Junior. / Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil. In: Brazilian Journal of Infectious Diseases. 2007 ; Vol. 11, No. 1. pp. 27-30.
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