Identification and characterization of Lbh, a novel conserved nuclear protein expressed during early limb and heart development

Karoline J. Briegel, Alexandra L. Joyner

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

We report the cloning, protein characterization, and expression of a novel vertebrate gene, termed Lbh (Limb-bud-and-heart), with a spatiotemporal expression pattern that marks embryologically significant domains in the developing limbs and heart. Lbh encodes a highly conserved nuclear protein, which in tissue culture cells possesses a transcriptional activator function. During limb development, expression of Lbh initiates in the ectoderm of the presumptive limb territory in the lateral body wall. As the limb buds appear, Lbh expression is restricted primarily to the distal ventral limb ectoderm and the apical ectodermal ridge, and overlaps in these ectodermal compartments with En1 and Fgf8 expression. During heart formation, Lbh is expressed as early as Nkx2.5 and dHand in the bilateral heart primordia, with the highest levels in the anterior promyocardium. After heart tube fusion and looping, Lbh expression is confined to the ventricular myocardium, with the highest intensity in the right ventricle and atrioventricular canal, as well as in the sinus venosus. Based on the molecular characteristics and the domain-specific expression pattern, it is possible that Lbh functions in synergy with other genes known to be required for heart and limb development.

Original languageEnglish (US)
Pages (from-to)291-304
Number of pages14
JournalDevelopmental Biology
Volume233
Issue number2
DOIs
StatePublished - May 15 2001
Externally publishedYes

Keywords

  • Cardiogenesis
  • Lbh
  • Limb development
  • Mouse embryogenesis
  • Nuclear
  • Pattern formation
  • Transcriptional activation
  • Xlcl2

ASJC Scopus subject areas

  • Developmental Biology

Fingerprint Dive into the research topics of 'Identification and characterization of Lbh, a novel conserved nuclear protein expressed during early limb and heart development'. Together they form a unique fingerprint.

  • Cite this