TY - JOUR
T1 - Identification and characterization of Lbh, a novel conserved nuclear protein expressed during early limb and heart development
AU - Briegel, Karoline J.
AU - Joyner, Alexandra L.
N1 - Funding Information:
We thank Se-Jin Lee, David Ron, Gail Martin, Andy McMahon, Kenneth Chien, and Eric Olson for reagents. We are grateful to C. Loomis and D. Yelon for comments on the manuscript. We also thank N. Dahmane, M. Horowitz, C. Jain, and the members of the Joyner lab for discussion and encouragement. This work was supported by a grant from the NIH. A.L.J. is an Investigator of the Howard Hughes Medical Institute. K.J.B. was a recipient of a long-term postdoctoral fellowship from the Human Frontiers of Science Organization (HFSPO) and is currently a Howard Hughes Research Associate.
PY - 2001/5/15
Y1 - 2001/5/15
N2 - We report the cloning, protein characterization, and expression of a novel vertebrate gene, termed Lbh (Limb-bud-and-heart), with a spatiotemporal expression pattern that marks embryologically significant domains in the developing limbs and heart. Lbh encodes a highly conserved nuclear protein, which in tissue culture cells possesses a transcriptional activator function. During limb development, expression of Lbh initiates in the ectoderm of the presumptive limb territory in the lateral body wall. As the limb buds appear, Lbh expression is restricted primarily to the distal ventral limb ectoderm and the apical ectodermal ridge, and overlaps in these ectodermal compartments with En1 and Fgf8 expression. During heart formation, Lbh is expressed as early as Nkx2.5 and dHand in the bilateral heart primordia, with the highest levels in the anterior promyocardium. After heart tube fusion and looping, Lbh expression is confined to the ventricular myocardium, with the highest intensity in the right ventricle and atrioventricular canal, as well as in the sinus venosus. Based on the molecular characteristics and the domain-specific expression pattern, it is possible that Lbh functions in synergy with other genes known to be required for heart and limb development.
AB - We report the cloning, protein characterization, and expression of a novel vertebrate gene, termed Lbh (Limb-bud-and-heart), with a spatiotemporal expression pattern that marks embryologically significant domains in the developing limbs and heart. Lbh encodes a highly conserved nuclear protein, which in tissue culture cells possesses a transcriptional activator function. During limb development, expression of Lbh initiates in the ectoderm of the presumptive limb territory in the lateral body wall. As the limb buds appear, Lbh expression is restricted primarily to the distal ventral limb ectoderm and the apical ectodermal ridge, and overlaps in these ectodermal compartments with En1 and Fgf8 expression. During heart formation, Lbh is expressed as early as Nkx2.5 and dHand in the bilateral heart primordia, with the highest levels in the anterior promyocardium. After heart tube fusion and looping, Lbh expression is confined to the ventricular myocardium, with the highest intensity in the right ventricle and atrioventricular canal, as well as in the sinus venosus. Based on the molecular characteristics and the domain-specific expression pattern, it is possible that Lbh functions in synergy with other genes known to be required for heart and limb development.
KW - Cardiogenesis
KW - Lbh
KW - Limb development
KW - Mouse embryogenesis
KW - Nuclear
KW - Pattern formation
KW - Transcriptional activation
KW - Xlcl2
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U2 - 10.1006/dbio.2001.0225
DO - 10.1006/dbio.2001.0225
M3 - Article
C2 - 11336496
AN - SCOPUS:0035873502
VL - 233
SP - 291
EP - 304
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -