Identification and characterization of Kaposi's sarcoma-associated herpesvirus K8.1 virion glycoprotein

Mengtao Li, John Mackey, Susan C. Czajak, Ronald Charles Desrosiers, Andrew A. Lackner, Jae U. Jung

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) has been consistently identified in Kaposi's sarcomas (KS), body cavity-based lymphomas (BCBL), and some forms of Castleman's disease. Previous serological tests with KS patient sera have detected lyric-cycle polypeptides from KSHV-infected BCBL cells. We have found that these polypeptides are predominantly encoded by the K8.1 open reading frame, which is present in the same genomic position as virion envelope glycoproteins of other gammaherpesviruses. The cDNA of K8.1 from BCBL-1 cells was found to encode a glycosylated protein with an apparent molecular mass of 37 kDa. K8.1 was found to be expressed during lytic KSHV replication in BCBL-1 cells and was localized on the surface of cells and virions. The results of immunofluorescence and immunoelectron microscopy suggest that KSHV acquires K8.1 protein on its virion surface during the process of budding at the plasma cell membrane. When KSHV K8.1 derived from mammalian cells was used as an antigen in immunoblot tests, antibodies to K8.1 were detected in 18 of 20 KS patients and in 0 of 10 KS-negative control subjects. These results demonstrate that the K8.1 gene encodes a KSHV virion- associated glycoprotein and suggest that antibodies to K8.1 may prove useful as contributory serological markers for infection by KSHV.

Original languageEnglish (US)
Pages (from-to)1341-1349
Number of pages9
JournalJournal of Virology
Volume73
Issue number2
StatePublished - 1999
Externally publishedYes

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Human herpesvirus 8
Human Herpesvirus 8
virion
Virion
glycoproteins
Glycoproteins
body cavities
Kaposi's Sarcoma
sarcoma
lymphoma
Lymphoma
cells
polypeptides
Cell Membrane
Giant Lymph Node Hyperplasia
Peptides
antibodies
Immunoelectron Microscopy
Antibodies
plasma cells

ASJC Scopus subject areas

  • Immunology

Cite this

Li, M., Mackey, J., Czajak, S. C., Desrosiers, R. C., Lackner, A. A., & Jung, J. U. (1999). Identification and characterization of Kaposi's sarcoma-associated herpesvirus K8.1 virion glycoprotein. Journal of Virology, 73(2), 1341-1349.

Identification and characterization of Kaposi's sarcoma-associated herpesvirus K8.1 virion glycoprotein. / Li, Mengtao; Mackey, John; Czajak, Susan C.; Desrosiers, Ronald Charles; Lackner, Andrew A.; Jung, Jae U.

In: Journal of Virology, Vol. 73, No. 2, 1999, p. 1341-1349.

Research output: Contribution to journalArticle

Li, M, Mackey, J, Czajak, SC, Desrosiers, RC, Lackner, AA & Jung, JU 1999, 'Identification and characterization of Kaposi's sarcoma-associated herpesvirus K8.1 virion glycoprotein', Journal of Virology, vol. 73, no. 2, pp. 1341-1349.
Li, Mengtao ; Mackey, John ; Czajak, Susan C. ; Desrosiers, Ronald Charles ; Lackner, Andrew A. ; Jung, Jae U. / Identification and characterization of Kaposi's sarcoma-associated herpesvirus K8.1 virion glycoprotein. In: Journal of Virology. 1999 ; Vol. 73, No. 2. pp. 1341-1349.
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