Identification and characterization of a novel matrix-degrading protease from hormone-dependent human breast cancer cells

Yuenian E. Shi, Jeff Torri, Lynn Yieh, Anton Wellstein, Marc E Lippman, Robert B. Dickson

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

A novel matrix-degrading enzyme was identified from human breast cancer cells. This enzyme appears as major gelatinase in hormone-dependent breast cancer cell lines and has as an apparent molecular mass of 80 KDa on gelatin zymography. The 80-kDa enzyme has a unique metal ion specificity. In addition to calcium ions, the gelatinolytic activity can be supported by manganese and/or magnesium. Unlike 92- and 72-kDa gelatinases and other known members of the metalloproteinase family, the 80-kDa protease is not activated by p-aminophenylmercuric acetate and its gelatinolytic activity is not inhibited by tissue inhibitor of metalloproteinase 2. It is active over the pH range 7.5-9.5 with an optimum at pH 8.5. The enzyme degrades gelatin and type IV collagen. The proteolytic activity of the enzyme is inhibited by EDTA and leupeptin. These unique features clearly distinguish the 80-kDa protease from the known 92-and 72-kDa gelatinases. The expression of 80-kDa enzyme can be detected in hormone-dependent human breast cancer cell lines in vitro and in tumors grown from these cells in athymic nude mice.

Original languageEnglish
Pages (from-to)1409-1415
Number of pages7
JournalCancer Research
Volume53
Issue number6
StatePublished - Mar 15 1993
Externally publishedYes

Fingerprint

Peptide Hydrolases
Hormones
Breast Neoplasms
Enzymes
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Gelatin
Nude Mice
Ions
Tissue Inhibitor of Metalloproteinase-2
Cell Line
Gelatinases
Collagen Type IV
Metalloproteases
Manganese
Edetic Acid
Magnesium
Acetates
Metals
Calcium

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Shi, Y. E., Torri, J., Yieh, L., Wellstein, A., Lippman, M. E., & Dickson, R. B. (1993). Identification and characterization of a novel matrix-degrading protease from hormone-dependent human breast cancer cells. Cancer Research, 53(6), 1409-1415.

Identification and characterization of a novel matrix-degrading protease from hormone-dependent human breast cancer cells. / Shi, Yuenian E.; Torri, Jeff; Yieh, Lynn; Wellstein, Anton; Lippman, Marc E; Dickson, Robert B.

In: Cancer Research, Vol. 53, No. 6, 15.03.1993, p. 1409-1415.

Research output: Contribution to journalArticle

Shi, YE, Torri, J, Yieh, L, Wellstein, A, Lippman, ME & Dickson, RB 1993, 'Identification and characterization of a novel matrix-degrading protease from hormone-dependent human breast cancer cells', Cancer Research, vol. 53, no. 6, pp. 1409-1415.
Shi YE, Torri J, Yieh L, Wellstein A, Lippman ME, Dickson RB. Identification and characterization of a novel matrix-degrading protease from hormone-dependent human breast cancer cells. Cancer Research. 1993 Mar 15;53(6):1409-1415.
Shi, Yuenian E. ; Torri, Jeff ; Yieh, Lynn ; Wellstein, Anton ; Lippman, Marc E ; Dickson, Robert B. / Identification and characterization of a novel matrix-degrading protease from hormone-dependent human breast cancer cells. In: Cancer Research. 1993 ; Vol. 53, No. 6. pp. 1409-1415.
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