Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection

Thomas S. Postler, Shara N. Pantry, Ronald Charles Desrosiers, Sankar Ghosh

Research output: Contribution to journalArticle

Abstract

Long non-coding RNAs (lncRNAs) are rapidly emerging as important regulators of a diverse array of cellular functions. Here, we describe a meta-analysis of two independent RNA-seq studies to identify lncRNAs that are differentially expressed upon HIV-1 infection. Only three lncRNA genes exhibited altered expression of ≥ 2-fold in HIV-1-infected cells. Of these, the uncharacterized lncRNA LINC00173 was chosen for further study. Both transcript variants of LINC00173 (lnc173 TSV1 and 2) could be detected by qPCR, localized predominantly to the nucleus and were reproducibly up-regulated during infection. Knock-out of the LINC00173 locus did not have detectable effects on HIV-1 replication. Interestingly, however, stimulation of Jurkat T cells with PMA/ionomycin resulted in a decrease of lnc173 expression, and Jurkat cells deficient for lnc173 on average expressed higher levels of specific cytokines than control cells. These data suggest that lnc173 may have a role in the regulation of cytokines in T cells.

Original languageEnglish (US)
Pages (from-to)30-39
Number of pages10
JournalVirology
Volume511
DOIs
StatePublished - Nov 1 2017

Fingerprint

Long Noncoding RNA
HIV Infections
HIV-1
Jurkat Cells
Cytokines
T-Lymphocytes
Ionomycin
Meta-Analysis
RNA
Infection
Genes

Keywords

  • Cytokine
  • Gene expression
  • HIV
  • Human immunodeficiency virus
  • lincRNA
  • lncRNA
  • Long non-coding RNA
  • RNA-seq

ASJC Scopus subject areas

  • Virology

Cite this

Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection. / Postler, Thomas S.; Pantry, Shara N.; Desrosiers, Ronald Charles; Ghosh, Sankar.

In: Virology, Vol. 511, 01.11.2017, p. 30-39.

Research output: Contribution to journalArticle

Postler, Thomas S. ; Pantry, Shara N. ; Desrosiers, Ronald Charles ; Ghosh, Sankar. / Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection. In: Virology. 2017 ; Vol. 511. pp. 30-39.
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