IADS, a decomposition product of DIDS activates a cation conductance in xenopus oocytes and human erythrocytes: New compound for the diagnosis of cystic fibrosis

Astrid Stumpf, Joana Almaca, Karl Kunzelmann, Kerstin Wenners-Epping, Stephan Huber, Johannes Haberle, Sabine Falk, Angelika Duebbers, Mike Walte, Hans Oberleithner, Hermann Schillers

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

DIDS (4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid) is a commonly used blocker of plasma membrane anion channels and transporters. We observed that DIDS undergoes decomposition while stored in DMSO (dimethyl sulfoxide) forming a biologically active compound. One decomposition product, called IADS, was identified and synthesized. Voltage-clamp and patch clamp experiments on Xenopus laevis oocytes and human erythrocytes revealed that IADS is able to activate a plasma membrane cation conductance in both cell types. Furthermore, we found that IADS induces hemolysis in red blood cells of healthy donors but fails to hemolyze erythrocytes of donors with cystic fibrosis. Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis.

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalCellular Physiology and Biochemistry
Volume18
Issue number4-5
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

Keywords

  • CFTR
  • Gadolinium
  • Hemolysis
  • Hydrolysis

ASJC Scopus subject areas

  • Physiology

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    Stumpf, A., Almaca, J., Kunzelmann, K., Wenners-Epping, K., Huber, S., Haberle, J., Falk, S., Duebbers, A., Walte, M., Oberleithner, H., & Schillers, H. (2006). IADS, a decomposition product of DIDS activates a cation conductance in xenopus oocytes and human erythrocytes: New compound for the diagnosis of cystic fibrosis. Cellular Physiology and Biochemistry, 18(4-5), 243-252. https://doi.org/10.1159/000097671