IADS, a decomposition product of DIDS activates a cation conductance in xenopus oocytes and human erythrocytes: New compound for the diagnosis of cystic fibrosis

Astrid Stumpf, Joana Almaca, Karl Kunzelmann, Kerstin Wenners-Epping, Stephan Huber, Johannes Haberle, Sabine Falk, Angelika Duebbers, Mike Walte, Hans Oberleithner, Hermann Schillers

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

DIDS (4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid) is a commonly used blocker of plasma membrane anion channels and transporters. We observed that DIDS undergoes decomposition while stored in DMSO (dimethyl sulfoxide) forming a biologically active compound. One decomposition product, called IADS, was identified and synthesized. Voltage-clamp and patch clamp experiments on Xenopus laevis oocytes and human erythrocytes revealed that IADS is able to activate a plasma membrane cation conductance in both cell types. Furthermore, we found that IADS induces hemolysis in red blood cells of healthy donors but fails to hemolyze erythrocytes of donors with cystic fibrosis. Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis.

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalCellular Physiology and Biochemistry
Volume18
Issue number4-5
DOIs
StatePublished - Dec 27 2006
Externally publishedYes

Fingerprint

Stilbenes
Xenopus
Cystic Fibrosis
Oocytes
Cations
Erythrocytes
Acids
Cell Membrane
Membrane Transport Proteins
Xenopus laevis
Hemolysis
Dimethyl Sulfoxide
Ion Channels
Routine Diagnostic Tests
Anions

Keywords

  • CFTR
  • Gadolinium
  • Hemolysis
  • Hydrolysis

ASJC Scopus subject areas

  • Physiology

Cite this

IADS, a decomposition product of DIDS activates a cation conductance in xenopus oocytes and human erythrocytes : New compound for the diagnosis of cystic fibrosis. / Stumpf, Astrid; Almaca, Joana; Kunzelmann, Karl; Wenners-Epping, Kerstin; Huber, Stephan; Haberle, Johannes; Falk, Sabine; Duebbers, Angelika; Walte, Mike; Oberleithner, Hans; Schillers, Hermann.

In: Cellular Physiology and Biochemistry, Vol. 18, No. 4-5, 27.12.2006, p. 243-252.

Research output: Contribution to journalArticle

Stumpf, A, Almaca, J, Kunzelmann, K, Wenners-Epping, K, Huber, S, Haberle, J, Falk, S, Duebbers, A, Walte, M, Oberleithner, H & Schillers, H 2006, 'IADS, a decomposition product of DIDS activates a cation conductance in xenopus oocytes and human erythrocytes: New compound for the diagnosis of cystic fibrosis', Cellular Physiology and Biochemistry, vol. 18, no. 4-5, pp. 243-252. https://doi.org/10.1159/000097671
Stumpf, Astrid ; Almaca, Joana ; Kunzelmann, Karl ; Wenners-Epping, Kerstin ; Huber, Stephan ; Haberle, Johannes ; Falk, Sabine ; Duebbers, Angelika ; Walte, Mike ; Oberleithner, Hans ; Schillers, Hermann. / IADS, a decomposition product of DIDS activates a cation conductance in xenopus oocytes and human erythrocytes : New compound for the diagnosis of cystic fibrosis. In: Cellular Physiology and Biochemistry. 2006 ; Vol. 18, No. 4-5. pp. 243-252.
@article{204005e448cf409d8b2795d1c446bb82,
title = "IADS, a decomposition product of DIDS activates a cation conductance in xenopus oocytes and human erythrocytes: New compound for the diagnosis of cystic fibrosis",
abstract = "DIDS (4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid) is a commonly used blocker of plasma membrane anion channels and transporters. We observed that DIDS undergoes decomposition while stored in DMSO (dimethyl sulfoxide) forming a biologically active compound. One decomposition product, called IADS, was identified and synthesized. Voltage-clamp and patch clamp experiments on Xenopus laevis oocytes and human erythrocytes revealed that IADS is able to activate a plasma membrane cation conductance in both cell types. Furthermore, we found that IADS induces hemolysis in red blood cells of healthy donors but fails to hemolyze erythrocytes of donors with cystic fibrosis. Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis.",
keywords = "CFTR, Gadolinium, Hemolysis, Hydrolysis",
author = "Astrid Stumpf and Joana Almaca and Karl Kunzelmann and Kerstin Wenners-Epping and Stephan Huber and Johannes Haberle and Sabine Falk and Angelika Duebbers and Mike Walte and Hans Oberleithner and Hermann Schillers",
year = "2006",
month = "12",
day = "27",
doi = "10.1159/000097671",
language = "English (US)",
volume = "18",
pages = "243--252",
journal = "Cellular Physiology and Biochemistry",
issn = "1015-8987",
publisher = "S. Karger AG",
number = "4-5",

}

TY - JOUR

T1 - IADS, a decomposition product of DIDS activates a cation conductance in xenopus oocytes and human erythrocytes

T2 - New compound for the diagnosis of cystic fibrosis

AU - Stumpf, Astrid

AU - Almaca, Joana

AU - Kunzelmann, Karl

AU - Wenners-Epping, Kerstin

AU - Huber, Stephan

AU - Haberle, Johannes

AU - Falk, Sabine

AU - Duebbers, Angelika

AU - Walte, Mike

AU - Oberleithner, Hans

AU - Schillers, Hermann

PY - 2006/12/27

Y1 - 2006/12/27

N2 - DIDS (4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid) is a commonly used blocker of plasma membrane anion channels and transporters. We observed that DIDS undergoes decomposition while stored in DMSO (dimethyl sulfoxide) forming a biologically active compound. One decomposition product, called IADS, was identified and synthesized. Voltage-clamp and patch clamp experiments on Xenopus laevis oocytes and human erythrocytes revealed that IADS is able to activate a plasma membrane cation conductance in both cell types. Furthermore, we found that IADS induces hemolysis in red blood cells of healthy donors but fails to hemolyze erythrocytes of donors with cystic fibrosis. Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis.

AB - DIDS (4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid) is a commonly used blocker of plasma membrane anion channels and transporters. We observed that DIDS undergoes decomposition while stored in DMSO (dimethyl sulfoxide) forming a biologically active compound. One decomposition product, called IADS, was identified and synthesized. Voltage-clamp and patch clamp experiments on Xenopus laevis oocytes and human erythrocytes revealed that IADS is able to activate a plasma membrane cation conductance in both cell types. Furthermore, we found that IADS induces hemolysis in red blood cells of healthy donors but fails to hemolyze erythrocytes of donors with cystic fibrosis. Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis.

KW - CFTR

KW - Gadolinium

KW - Hemolysis

KW - Hydrolysis

UR - http://www.scopus.com/inward/record.url?scp=33845663195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845663195&partnerID=8YFLogxK

U2 - 10.1159/000097671

DO - 10.1159/000097671

M3 - Article

C2 - 17167229

AN - SCOPUS:33845663195

VL - 18

SP - 243

EP - 252

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

SN - 1015-8987

IS - 4-5

ER -