Hypoxic Stress Decreases c-Myc Protein Stability in Cardiac Progenitor Cells Inducing Quiescence and Compromising Their Proliferative and Vasculogenic Potential

Michael A. Bellio, Mariana T. Pinto, Victoria Florea, Paola A. Barrios, Christy N. Taylor, Ariel B. Brown, Courtney Lamondin, Joshua Hare, Ivonne H Schulman, Claudia D Rodrigues

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cardiac progenitor cells (CPCs) have been shown to promote cardiac regeneration and improve heart function. However, evidence suggests that their regenerative capacity may be limited in conditions of severe hypoxia. Elucidating the mechanisms involved in CPC protection against hypoxic stress is essential to maximize their cardioprotective and therapeutic potential. We investigated the effects of hypoxic stress on CPCs and found significant reduction in proliferation and impairment of vasculogenesis, which were associated with induction of quiescence, as indicated by accumulation of cells in the G0-phase of the cell cycle and growth recovery when cells were returned to normoxia. Induction of quiescence was associated with a decrease in the expression of c-Myc through mechanisms involving protein degradation and upregulation of p21. Inhibition of c-Myc mimicked the effects of severe hypoxia on CPC proliferation, also triggering quiescence. Surprisingly, these effects did not involve changes in p21 expression, indicating that other hypoxia-activated factors may induce p21 in CPCs. Our results suggest that hypoxic stress compromises CPC function by inducing quiescence in part through downregulation of c-Myc. In addition, we found that c-Myc is required to preserve CPC growth, suggesting that modulation of pathways downstream of it may re-activate CPC regenerative potential under ischemic conditions.

Original languageEnglish (US)
Article number9702
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Proto-Oncogene Proteins c-myc
Protein Stability
Stem Cells
Cell Cycle Resting Phase
Cytoprotection
Growth
Proteolysis
Regeneration
Cell Cycle
Up-Regulation
Down-Regulation
Cell Proliferation

ASJC Scopus subject areas

  • General

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Hypoxic Stress Decreases c-Myc Protein Stability in Cardiac Progenitor Cells Inducing Quiescence and Compromising Their Proliferative and Vasculogenic Potential. / Bellio, Michael A.; Pinto, Mariana T.; Florea, Victoria; Barrios, Paola A.; Taylor, Christy N.; Brown, Ariel B.; Lamondin, Courtney; Hare, Joshua; Schulman, Ivonne H; Rodrigues, Claudia D.

In: Scientific Reports, Vol. 7, No. 1, 9702, 01.12.2017.

Research output: Contribution to journalArticle

Bellio, Michael A. ; Pinto, Mariana T. ; Florea, Victoria ; Barrios, Paola A. ; Taylor, Christy N. ; Brown, Ariel B. ; Lamondin, Courtney ; Hare, Joshua ; Schulman, Ivonne H ; Rodrigues, Claudia D. / Hypoxic Stress Decreases c-Myc Protein Stability in Cardiac Progenitor Cells Inducing Quiescence and Compromising Their Proliferative and Vasculogenic Potential. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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abstract = "Cardiac progenitor cells (CPCs) have been shown to promote cardiac regeneration and improve heart function. However, evidence suggests that their regenerative capacity may be limited in conditions of severe hypoxia. Elucidating the mechanisms involved in CPC protection against hypoxic stress is essential to maximize their cardioprotective and therapeutic potential. We investigated the effects of hypoxic stress on CPCs and found significant reduction in proliferation and impairment of vasculogenesis, which were associated with induction of quiescence, as indicated by accumulation of cells in the G0-phase of the cell cycle and growth recovery when cells were returned to normoxia. Induction of quiescence was associated with a decrease in the expression of c-Myc through mechanisms involving protein degradation and upregulation of p21. Inhibition of c-Myc mimicked the effects of severe hypoxia on CPC proliferation, also triggering quiescence. Surprisingly, these effects did not involve changes in p21 expression, indicating that other hypoxia-activated factors may induce p21 in CPCs. Our results suggest that hypoxic stress compromises CPC function by inducing quiescence in part through downregulation of c-Myc. In addition, we found that c-Myc is required to preserve CPC growth, suggesting that modulation of pathways downstream of it may re-activate CPC regenerative potential under ischemic conditions.",
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