Hypothermic storage and cryopreservation of cartilage. An experimental study

Theodore Malinin, J. L. Wagner, J. C. Pita, H. Lo

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Osteochondral autografts of femoral condyles in dogs were frozen at different cooling velocities after exposure to either glycerol or dimethyl sulfoxide to determine the freezing regimen best suited for preservation of intact cartilage. Autografts were also subjected to hypothermic storage in tissue culture media for ascertainment of how long they can be stored under these conditions. Autografts maintained in vitro under various conditions of storage were examined after transplantation. Autografts were chosen for this portion of the investigation in order to study the effects of storage uncomplicated by immunologic interactions. No differences were found between the cryoprotective actions of glycerol and dimethyl sulfoxide. The freezing rate that produced the least damage in the cartilage was 2°/min. Cartilage survived ten days of hypothermic storage in tissue culture medium. Allografts frozen by the standard method of glycerolization and cooling at 1°/min were also studied. These showed eventual loss of chondrocytes and conversion of hyaline cartilage to fibrocartilage.

Original languageEnglish
Pages (from-to)15-26
Number of pages12
JournalClinical Orthopaedics and Related Research
VolumeNO. 197
StatePublished - Jan 1 1985

Fingerprint

Cryopreservation
Autografts
Cartilage
Dimethyl Sulfoxide
Glycerol
Freezing
Culture Media
Fibrocartilage
Hyaline Cartilage
Chondrocytes
Thigh
Allografts
Transplantation
Dogs
Bone and Bones

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Hypothermic storage and cryopreservation of cartilage. An experimental study. / Malinin, Theodore; Wagner, J. L.; Pita, J. C.; Lo, H.

In: Clinical Orthopaedics and Related Research, Vol. NO. 197, 01.01.1985, p. 15-26.

Research output: Contribution to journalArticle

Malinin, Theodore ; Wagner, J. L. ; Pita, J. C. ; Lo, H. / Hypothermic storage and cryopreservation of cartilage. An experimental study. In: Clinical Orthopaedics and Related Research. 1985 ; Vol. NO. 197. pp. 15-26.
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