Hyperinnervation of the airways in transgenic mice overexpressing nerve growth factor

Gary W. Hoyle, Regina M Graham, Jeffrey B. Finkelstein, Kim Phuong Thi Nguyen, David Gozal, Mitchell Friedman

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

Neuropeptides released from sensory nerve endings are potential mediators of airway inflammation in asthma and lung injury induced by inhalation of respiratory irritants. To develop an in vivo model for assessing the contribution of neurogenic inflammation in these processes, we have generated transgenic mice with altered innervation of the lung. To generate mice with an increased innervation of the airways, we placed the gene that encodes nerve growth factor (NGF) under control of the lung-specific Clara-cell secretory protein (CCSP) promoter. Two lineages of CCSP-NGF transgenic mice overexpressed NGF in the lung and developed a hyperinnervation of the airways. Immunohistochemistry for substance P, a substance P enzyme immunoassay, and catecholamine histofluorescence indicated that both tachykinin-containing sensory fibers and sympathetic fibers were increased around the airways of CCSP-NGF mice. Treatment of CCSP-NGF mice with the sympathetic-specific neurotoxin 6-hydroxydopamine (6-OHDA) eliminated the sympathetic component of the airway innervation, leaving a specific hyperinnervation by tachykinin-containing sensory fibers. CCSP-NGF mice were more sensitive than normal mice to capsaicin-induced increases in respiratory system resistance, demonstrating that the increased sensory innervation led to a change in airway function. We conclude that NGF overexpression from a lung-specific promoter produces anatomic and functional changes in lung innervation, and that CCSP-NGF mice will be useful for studying the role of neurogenic inflammation in airway disease.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume18
Issue number2
StatePublished - 1998
Externally publishedYes

Fingerprint

Uteroglobin
Nerve Growth Factor
Transgenic Mice
Lung
Neurogenic Inflammation
Tachykinins
Oxidopamine
Substance P
Fibers
Respiratory system
Adrenergic Fibers
Inflammation Mediators
Irritants
Capsaicin
Neurotoxins
Lung Injury
Sensory Receptor Cells
Neuropeptides
Immunoenzyme Techniques
Respiratory System

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Hoyle, G. W., Graham, R. M., Finkelstein, J. B., Nguyen, K. P. T., Gozal, D., & Friedman, M. (1998). Hyperinnervation of the airways in transgenic mice overexpressing nerve growth factor. American Journal of Respiratory Cell and Molecular Biology, 18(2), 149-157.

Hyperinnervation of the airways in transgenic mice overexpressing nerve growth factor. / Hoyle, Gary W.; Graham, Regina M; Finkelstein, Jeffrey B.; Nguyen, Kim Phuong Thi; Gozal, David; Friedman, Mitchell.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 18, No. 2, 1998, p. 149-157.

Research output: Contribution to journalArticle

Hoyle, GW, Graham, RM, Finkelstein, JB, Nguyen, KPT, Gozal, D & Friedman, M 1998, 'Hyperinnervation of the airways in transgenic mice overexpressing nerve growth factor', American Journal of Respiratory Cell and Molecular Biology, vol. 18, no. 2, pp. 149-157.
Hoyle, Gary W. ; Graham, Regina M ; Finkelstein, Jeffrey B. ; Nguyen, Kim Phuong Thi ; Gozal, David ; Friedman, Mitchell. / Hyperinnervation of the airways in transgenic mice overexpressing nerve growth factor. In: American Journal of Respiratory Cell and Molecular Biology. 1998 ; Vol. 18, No. 2. pp. 149-157.
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