Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo

Tatyana N. Milovanova, Veena M. Bhopale, Elena M. Sorokina, Jonni S. Moore, Thomas K. Hunt, Martin Hauer-Jensen, Omaida C Velazquez, Stephen R. Thom

Research output: Contribution to journalArticle

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Abstract

We hypothesized that oxidative stress from hyperbaric oxygen (HBO 2, 2.8 ATA for 90 min daily) exerts a trophic effect on vasculogenic stem cells. In a mouse model, circulating stem/progenitor cell (SPC) recruitment and differentiation in subcutaneous Matrigel were stimulated by HBO2 and by a physiological oxidative stressor, lactate. In combination, HBO 2 and lactate had additive effects. Vascular channels lined by CD34+ SPCs were identified. HBO2 and lactate accelerated channel development, cell differentiation based on surface marker expression, and cell cycle entry. CD34+ SPCs exhibited increases in thioredoxin-1 (Trx1), Trx reductase, hypoxia-inducible factors (HIF)-1, -2, and -3, phosphorylated mitogen-activated protein kinases, vascular endothelial growth factor, and stromal cell-derived factor-1. Cell recruitment to Matrigel and protein synthesis responses were abrogated by N-acetyl cysteine, dithioerythritol, oxamate, apocynin, U-0126, neutralizing anti-vascular endothelial growth factor, or antistromal cell-derived factor-1 antibodies, and small inhibitory RNA to Trx reductase, lactate dehydrogenase, gp91 phox, HIF-1 or -2, and in mice conditionally null for HIF-1 in myeloid cells. By causing an oxidative stress, HBO2 activates a physiological redox-active autocrine loop in SPCs that stimulates vasculogenesis. Thioredoxin system activation leads to elevations in HIF-1 and -2, followed by synthesis of HIF-dependent growth factors. HIF-3 has a negative impact on SPCs.

Original languageEnglish
Pages (from-to)711-728
Number of pages18
JournalJournal of Applied Physiology
Volume106
Issue number2
DOIs
StatePublished - Feb 1 2009

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Hypoxia-Inducible Factor 1
Cell Differentiation
Stem Cells
Oxygen
Lactic Acid
Growth
Vascular Endothelial Growth Factor A
Thioredoxin Reductase 1
Oxidative Stress
Dithioerythritol
Chemokine CXCL12
Thioredoxins
Mitogen-Activated Protein Kinase 3
Myeloid Cells
L-Lactate Dehydrogenase
Oxidation-Reduction
Cysteine
Blood Vessels
Intercellular Signaling Peptides and Proteins
Cell Cycle

Keywords

  • CD34
  • Hypoxia inducible factor-1
  • Hypoxia inducible factor-2
  • Hypoxia inducible factor-3
  • Mitogen-activated protein kinase
  • Stromal cell-derived factor-1
  • Thioredoxin
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Milovanova, T. N., Bhopale, V. M., Sorokina, E. M., Moore, J. S., Hunt, T. K., Hauer-Jensen, M., ... Thom, S. R. (2009). Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. Journal of Applied Physiology, 106(2), 711-728. https://doi.org/10.1152/japplphysiol.91054.2008

Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. / Milovanova, Tatyana N.; Bhopale, Veena M.; Sorokina, Elena M.; Moore, Jonni S.; Hunt, Thomas K.; Hauer-Jensen, Martin; Velazquez, Omaida C; Thom, Stephen R.

In: Journal of Applied Physiology, Vol. 106, No. 2, 01.02.2009, p. 711-728.

Research output: Contribution to journalArticle

Milovanova, TN, Bhopale, VM, Sorokina, EM, Moore, JS, Hunt, TK, Hauer-Jensen, M, Velazquez, OC & Thom, SR 2009, 'Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo', Journal of Applied Physiology, vol. 106, no. 2, pp. 711-728. https://doi.org/10.1152/japplphysiol.91054.2008
Milovanova TN, Bhopale VM, Sorokina EM, Moore JS, Hunt TK, Hauer-Jensen M et al. Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. Journal of Applied Physiology. 2009 Feb 1;106(2):711-728. https://doi.org/10.1152/japplphysiol.91054.2008
Milovanova, Tatyana N. ; Bhopale, Veena M. ; Sorokina, Elena M. ; Moore, Jonni S. ; Hunt, Thomas K. ; Hauer-Jensen, Martin ; Velazquez, Omaida C ; Thom, Stephen R. / Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. In: Journal of Applied Physiology. 2009 ; Vol. 106, No. 2. pp. 711-728.
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