Hydrogen Peroxide Stimulates Exosomal Cathepsin B Regulation of the Receptor for Advanced Glycation End-Products (RAGE)

Charles A. Downs, Viet D. Dang, Nicholle M. Johnson, Nancy D. Denslow, Abdel A. Alli

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Exosomes are nano-sized vesicles that are secreted into the extracellular environment. These vesicles contain various biological effector molecules that can regulate intracellular signaling pathways in recipient cells. The aim of this study was to examine a correlation between exosomal cathepsin B activity and the receptor for advanced glycation end-products (RAGE). Type 1 alveolar epithelial (R3/1) cells were treated with or without hydrogen peroxide and exosomes isolated from the cell conditioned media were characterized by NanoSight analysis. Lipidomic and proteomic analysis showed exosomes released from R3/1 cells exposed to oxidative stress induced by hydrogen peroxide or vehicle differ in their lipid and protein content, respectively. Cathepsin B activity was detected in exosomes isolated from hydrogen peroxide treated cells. The mRNA and protein expression of RAGE increased in cultured R3/1 cells treated with exosomes containing active cathepsin B while depletion of exosomal cathepsin B attenuated RAGE mRNA and protein expression. These results suggest exosomal cathepsin B regulates RAGE in type 1 alveolar cells under conditions of oxidative stress. J. Cell. Biochem. 119: 599–606, 2018.

Original languageEnglish (US)
Pages (from-to)599-606
Number of pages8
JournalJournal of cellular biochemistry
Volume119
Issue number1
DOIs
StatePublished - Jan 2018
Externally publishedYes

Keywords

  • EXOSOMES
  • LIPIDOMICS
  • PROTEOMICS

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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