Hydrogen peroxide promotes injury-induced peripheral sensory axon regeneration in the zebrafish skin

Sandra Rieger, Alvaro Sagasti

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Functional recovery from cutaneous injury requires not only the healing and regeneration of skin cells but also reinnervation of the skin by somatosensory peripheral axon endings. To investigate how sensory axon regeneration and wound healing are coordinated, we amputated the caudal fins of zebrafish larvae and imaged somatosensory axon behavior. Fin amputation strongly promoted the regeneration of nearby sensory axons, an effect that could be mimicked by ablating a few keratinocytes anywhere in the body. Since injury produces the reactive oxygen species hydrogen peroxide (H2O2) near wounds, we tested whether H2O2 influences cutaneous axon regeneration. Exposure of zebrafish larvae to sublethal levels of exogenous H2O2 promoted growth of severed axons in the absence of keratinocyte injury, and inhibiting H2O2 production blocked the axon growth-promoting effects of fin amputation and keratinocyte ablation. Thus, H2O2 signaling helps coordinate wound healing with peripheral sensory axon reinnervation of the skin.

Original languageEnglish (US)
Article numbere1000621
JournalPLoS biology
Volume9
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint

Dive into the research topics of 'Hydrogen peroxide promotes injury-induced peripheral sensory axon regeneration in the zebrafish skin'. Together they form a unique fingerprint.

Cite this