Hydrogen peroxide localization in experimental optic neuritis

John Guy, E. A. Ellis, N. A. Rao

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The association of reactive oxygen species to altered permeability of the blood-brain barrier in acute experimental encephalomyelitis was investigated by ultrastructural cytochemical localization of hydrogen peroxide (H2O2) to sites in the optic nerve previously identified by extravasation of intravascular horseradish peroxidase. Using a modified cerium method, we found electron-dense cerium-derived H2O2 reaction product was localized to the perivascular space at the lamina retinalis, lamina choroidalis, and lamina scleralis. In the optic nerve head, electron-dense reaction product was observed in the presence of intravascular leukocytes, although adjacent perivascular and interstitial inflammatory cells at this site were scant. In the myelinated retrobulbar optic nerve, cerium-derived H2O2 reaction product was seen in the intravascular space of blood vessels and surrounding perivascular and interstitial foci of inflammatory cells. Reaction product was also observed in the extracellular space adjacent to the plasmalemma of axons and glial cells in the optic nerve head and retrobulbar nerve. the perivascular and intravascular distribution of cerium-derived reaction product suggests that H2O2 may play a role in the pathogenesis of altered vascular permeability in experimental optic neuritis and supports our previous observations of suppression of blood-brain barrier permeability by detoxification of H2O2 with the exogenous administration of antioxidant enzymes.

Original languageEnglish
Pages (from-to)1614-1621
Number of pages8
JournalArchives of Ophthalmology
Volume108
Issue number11
StatePublished - Nov 26 1990
Externally publishedYes

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Cerium
Optic Neuritis
Hydrogen Peroxide
Optic Disk
Optic Nerve
Blood-Brain Barrier
Permeability
Electrons
Encephalomyelitis
Extracellular Space
Capillary Permeability
Horseradish Peroxidase
Neuroglia
Blood Vessels
Axons
Reactive Oxygen Species
Leukocytes
Antioxidants
Enzymes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Hydrogen peroxide localization in experimental optic neuritis. / Guy, John; Ellis, E. A.; Rao, N. A.

In: Archives of Ophthalmology, Vol. 108, No. 11, 26.11.1990, p. 1614-1621.

Research output: Contribution to journalArticle

Guy, J, Ellis, EA & Rao, NA 1990, 'Hydrogen peroxide localization in experimental optic neuritis', Archives of Ophthalmology, vol. 108, no. 11, pp. 1614-1621.
Guy, John ; Ellis, E. A. ; Rao, N. A. / Hydrogen peroxide localization in experimental optic neuritis. In: Archives of Ophthalmology. 1990 ; Vol. 108, No. 11. pp. 1614-1621.
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