Hybrid Nanoparticles Based on Organized Protein Immobilization on Fullerenes

Pramod Nednoor, Marcello Capaccio, Vasilis G. Gavalas, Mark S. Meier, John E. Anthony, Leonidas G. Bachas

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Nanoscale carbon materials (i.e., fullerenes and nanotubes) are an attractive platform for applications in biotransformations and biosensors. The interesting properties displayed by nanoparticles demand new strategies for the manipulation of these materials on the nanoscale. Controlled modification of their surface with biomolecules is required to fully realize their potential in bionanotechnology. In this work, immobilization of a fullerene derivative with a mutant subtilisin is demonstrated, and the effect of the fullerene on the protein activity is determined. The fullerene-conjugated enzyme had improved catalytic properties in comparison to subtilisin immobilized on nonporous silica. Further, the pH profile of free and fullerene-conjugated subtilisin were almost identical.

Original languageEnglish (US)
Pages (from-to)12-15
Number of pages4
JournalBioconjugate Chemistry
Volume15
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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