Hyaluronate coating enhances the delivery and biocompatibility of gold nanoparticles

Bedia Begum Karakocak, Jue Liang, Pratim Biswas, Nathan Ravi

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

For targeted delivery with nanoparticles (NPs) as drug carriers, it is imperative that the NPs are internalized into the targeted cell. Surface properties of NPs influence their interactions with cells. We examined the responses of retinal pigment epithelial cells, NIH 3T3 fibroblast cells, and Chinese hamster ovary cells to gold nanoparticles (Au NPs) in their nascent form as well as coated with end-thiolated hyaluronate (HS-HA). The grafting density of HS-HA on Au NPs was calculated based on total organic carbon measurements and thermal gravimetric analysis. We imaged the intracellular NPs by 3D confocal microscopy. We quantified viability and generation of reactive oxygen species (ROS) of the cells to Au NPs and monitored cell-surface attachment via electrical cell-substrate impedance sensing. The results confirmed that receptors on cell surfaces, for HA, are critical in internalizing HS-HA-Au NPs, and HA may mitigate ROS pathways known to lead to cell death. The 50- and 100-nm HS-HA-Au NPs were able to enter the cells; however, their nascent forms could not. This study shows that the delivery of larger Au NPs is enhanced with HS-HA coating and illustrates the potential of HA-coated NPs as a drug delivery agent for inflamed, proliferating, and cancer cells that express CD44 receptors.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalCarbohydrate Polymers
Volume186
DOIs
StatePublished - Apr 15 2018
Externally publishedYes

Keywords

  • 3D confocal
  • CD44
  • Gold nanoparticles
  • Internalization
  • Thiolated-hyaluronate

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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