hUNC93B1: A novel human gene representing a new gene family and encoding an unc-93-like protein

Vladimir I. Kashuba, Alexei I. Protopopov, Sergei M. Kvasha, Rinat Z. Gizatullin, Claes R Wahlestedt, Lev L. Kisselev, George Klein, Eugene R. Zabarovsky

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have identified a novel human gene UNC93B1 encoding a protein related to unc-93 of Caenorhabditis elegans. The combined sequence derived from several cDNA clones is 2282 bp and comparison with genomic sequence shows that the gene contains 11 exons. The longest open reading frame encodes a deduced sequence of 597 amino acids. Homology analysis shows that the hUNC93B1 gene is highly conserved and related to sequences in Arabidopsis thaliana, C. elegans, Drosophila melanogaster, chicken and mouse. Structural analysis of the deduced amino acid sequence of hUNC93B1 points to possible existence of multiple membrane-spanning domains. hUNC93B1 protein also displays some similarities to the bacterial ABC-2 type transporter signature and to ion transporters of Deinococcus radiodurans and Helicobacter pylori. As revealed by Northern analysis, the level of expression varies significantly between tissues, with the highest level detected in the heart. The gene was mapped to chromosomal band 11q13 by fluorescence in situ hybridization. We suggest that this gene is a member of a novel hUNC93B-related gene family.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalGene
Volume283
Issue number1-2
DOIs
StatePublished - Jan 23 2002
Externally publishedYes

Fingerprint

Genes
Proteins
Caenorhabditis elegans
Deinococcus
ATP-Binding Cassette Transporters
Protein Sequence Analysis
Drosophila melanogaster
Fluorescence In Situ Hybridization
Arabidopsis
Helicobacter pylori
Open Reading Frames
Amino Acid Sequence
Chickens
Exons
Complementary DNA
Clone Cells
Ions
Membranes

Keywords

  • Conserved gene probes
  • Gene mapping
  • Molecular cloning
  • Multigene family
  • NotI-linking clone
  • Transporter protein

ASJC Scopus subject areas

  • Genetics

Cite this

Kashuba, V. I., Protopopov, A. I., Kvasha, S. M., Gizatullin, R. Z., Wahlestedt, C. R., Kisselev, L. L., ... Zabarovsky, E. R. (2002). hUNC93B1: A novel human gene representing a new gene family and encoding an unc-93-like protein. Gene, 283(1-2), 209-217. https://doi.org/10.1016/S0378-1119(01)00856-3

hUNC93B1 : A novel human gene representing a new gene family and encoding an unc-93-like protein. / Kashuba, Vladimir I.; Protopopov, Alexei I.; Kvasha, Sergei M.; Gizatullin, Rinat Z.; Wahlestedt, Claes R; Kisselev, Lev L.; Klein, George; Zabarovsky, Eugene R.

In: Gene, Vol. 283, No. 1-2, 23.01.2002, p. 209-217.

Research output: Contribution to journalArticle

Kashuba, VI, Protopopov, AI, Kvasha, SM, Gizatullin, RZ, Wahlestedt, CR, Kisselev, LL, Klein, G & Zabarovsky, ER 2002, 'hUNC93B1: A novel human gene representing a new gene family and encoding an unc-93-like protein', Gene, vol. 283, no. 1-2, pp. 209-217. https://doi.org/10.1016/S0378-1119(01)00856-3
Kashuba VI, Protopopov AI, Kvasha SM, Gizatullin RZ, Wahlestedt CR, Kisselev LL et al. hUNC93B1: A novel human gene representing a new gene family and encoding an unc-93-like protein. Gene. 2002 Jan 23;283(1-2):209-217. https://doi.org/10.1016/S0378-1119(01)00856-3
Kashuba, Vladimir I. ; Protopopov, Alexei I. ; Kvasha, Sergei M. ; Gizatullin, Rinat Z. ; Wahlestedt, Claes R ; Kisselev, Lev L. ; Klein, George ; Zabarovsky, Eugene R. / hUNC93B1 : A novel human gene representing a new gene family and encoding an unc-93-like protein. In: Gene. 2002 ; Vol. 283, No. 1-2. pp. 209-217.
@article{b6484de60f764cd589f7a1db4540dc9a,
title = "hUNC93B1: A novel human gene representing a new gene family and encoding an unc-93-like protein",
abstract = "We have identified a novel human gene UNC93B1 encoding a protein related to unc-93 of Caenorhabditis elegans. The combined sequence derived from several cDNA clones is 2282 bp and comparison with genomic sequence shows that the gene contains 11 exons. The longest open reading frame encodes a deduced sequence of 597 amino acids. Homology analysis shows that the hUNC93B1 gene is highly conserved and related to sequences in Arabidopsis thaliana, C. elegans, Drosophila melanogaster, chicken and mouse. Structural analysis of the deduced amino acid sequence of hUNC93B1 points to possible existence of multiple membrane-spanning domains. hUNC93B1 protein also displays some similarities to the bacterial ABC-2 type transporter signature and to ion transporters of Deinococcus radiodurans and Helicobacter pylori. As revealed by Northern analysis, the level of expression varies significantly between tissues, with the highest level detected in the heart. The gene was mapped to chromosomal band 11q13 by fluorescence in situ hybridization. We suggest that this gene is a member of a novel hUNC93B-related gene family.",
keywords = "Conserved gene probes, Gene mapping, Molecular cloning, Multigene family, NotI-linking clone, Transporter protein",
author = "Kashuba, {Vladimir I.} and Protopopov, {Alexei I.} and Kvasha, {Sergei M.} and Gizatullin, {Rinat Z.} and Wahlestedt, {Claes R} and Kisselev, {Lev L.} and George Klein and Zabarovsky, {Eugene R.}",
year = "2002",
month = "1",
day = "23",
doi = "10.1016/S0378-1119(01)00856-3",
language = "English",
volume = "283",
pages = "209--217",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - hUNC93B1

T2 - A novel human gene representing a new gene family and encoding an unc-93-like protein

AU - Kashuba, Vladimir I.

AU - Protopopov, Alexei I.

AU - Kvasha, Sergei M.

AU - Gizatullin, Rinat Z.

AU - Wahlestedt, Claes R

AU - Kisselev, Lev L.

AU - Klein, George

AU - Zabarovsky, Eugene R.

PY - 2002/1/23

Y1 - 2002/1/23

N2 - We have identified a novel human gene UNC93B1 encoding a protein related to unc-93 of Caenorhabditis elegans. The combined sequence derived from several cDNA clones is 2282 bp and comparison with genomic sequence shows that the gene contains 11 exons. The longest open reading frame encodes a deduced sequence of 597 amino acids. Homology analysis shows that the hUNC93B1 gene is highly conserved and related to sequences in Arabidopsis thaliana, C. elegans, Drosophila melanogaster, chicken and mouse. Structural analysis of the deduced amino acid sequence of hUNC93B1 points to possible existence of multiple membrane-spanning domains. hUNC93B1 protein also displays some similarities to the bacterial ABC-2 type transporter signature and to ion transporters of Deinococcus radiodurans and Helicobacter pylori. As revealed by Northern analysis, the level of expression varies significantly between tissues, with the highest level detected in the heart. The gene was mapped to chromosomal band 11q13 by fluorescence in situ hybridization. We suggest that this gene is a member of a novel hUNC93B-related gene family.

AB - We have identified a novel human gene UNC93B1 encoding a protein related to unc-93 of Caenorhabditis elegans. The combined sequence derived from several cDNA clones is 2282 bp and comparison with genomic sequence shows that the gene contains 11 exons. The longest open reading frame encodes a deduced sequence of 597 amino acids. Homology analysis shows that the hUNC93B1 gene is highly conserved and related to sequences in Arabidopsis thaliana, C. elegans, Drosophila melanogaster, chicken and mouse. Structural analysis of the deduced amino acid sequence of hUNC93B1 points to possible existence of multiple membrane-spanning domains. hUNC93B1 protein also displays some similarities to the bacterial ABC-2 type transporter signature and to ion transporters of Deinococcus radiodurans and Helicobacter pylori. As revealed by Northern analysis, the level of expression varies significantly between tissues, with the highest level detected in the heart. The gene was mapped to chromosomal band 11q13 by fluorescence in situ hybridization. We suggest that this gene is a member of a novel hUNC93B-related gene family.

KW - Conserved gene probes

KW - Gene mapping

KW - Molecular cloning

KW - Multigene family

KW - NotI-linking clone

KW - Transporter protein

UR - http://www.scopus.com/inward/record.url?scp=0037160535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037160535&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(01)00856-3

DO - 10.1016/S0378-1119(01)00856-3

M3 - Article

C2 - 11867227

AN - SCOPUS:0037160535

VL - 283

SP - 209

EP - 217

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -