Human umbilical cord blood cells in infarcted rats

V. Pinho-Ribeiro, A. C.V. Maia, J. P.S. Werneck-de-Castro, P. F. Oliveira, R. C.S. Goldenberg, A. C. Campos De Carvalho

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Therapy with bone marrow-derived cells has been used in ischemic patients with reported success. The aim of this study was to determine the therapeutic efficacy of fresh and frozen human umbilical cord blood cells (hUCB) in Wistar rats submitted to permanent occlusion of the left coronary artery. Three hours after myocardial infarction, 2 x 107 hUCB cells or vehicle were administered by intramyocardial injection. The animals were divided into five groups: control (N = 10), sham operated (N = 10), infarcted that received vehicle (N = 9), infarcted treated with cryopreserved hUCB (N = 7), and infarcted treated with fresh hUCB (N = 5). Cardiac function was evaluated by electrocardiogram (ECG) and echocardiogram (ECHO) before cell therapy, and by ECG, ECHO, cardiopulmonary test, and left ventricular pressure measurements 3 weeks later. After 3 weeks, both groups treated with hUCB still had Q wave present in L1, âQRS >90° and reduced shortening fraction (less than 50%). In addition, cardiac indexes of left ventricular contractility and relaxation were 5484 ± 875 and -4032 ± 643 mmHg (cryopreserved hUCB) and 4585 ± 955 and -2862 ± 590 mmHg (fresh hUCB), respectively. These values were not statistically different from those of saline-treated animals. Cardiopulmonary exercise test profile was typical of infarcted hearts; exercise time was about 14 min and maximal VO2 was 24.77 ± 5.00 mL·kg-1·min-1. These data show that hUCB therapy did not improve the cardiac function of infarcted animals or prevent cardiac remodeling.

Original languageEnglish (US)
Pages (from-to)290-296
Number of pages7
JournalBrazilian Journal of Medical and Biological Research
Volume43
Issue number3
DOIs
StatePublished - Mar 2010
Externally publishedYes

Keywords

  • Acute myocardial infarction
  • Cell therapy
  • Echocardiogram
  • Electrocardiogram
  • Fresh and frozen human umbilical cord blood

ASJC Scopus subject areas

  • Biophysics
  • Neuroscience(all)
  • Biochemistry
  • Physiology
  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Cell Biology

Fingerprint

Dive into the research topics of 'Human umbilical cord blood cells in infarcted rats'. Together they form a unique fingerprint.

Cite this