Human T lymphocytes and myeloid colony forming cells share common antigen

J. W. Chiao, Rajendra Pahwa, R. A. Good

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Human peripheral blood T lymphocyte antigen has been detected by goat antisera raised to thymocytes of Rhesus monkey. An absorbed antiserum reacted in complement-mediated cytotoxicity reactions and absorption experiments with isolated human peripheral blood T lymphocytes, thymocytes, cells of T lymphocyte line, but not with B lymphocytes or cells of B lymphocyte line. Anti-T lymphocyte antibodies blocked the T lymphocyte rosette formation with sheep erythrocytes. By cytotoxicity test an average of 85% cells of unfractionated lymphocyte preparations and 98% cells of isolated T lymphocyte preparations were positive for the antigen. Peripheral blood granulocytes and monocytes were shown to lack the antigen by cytotoxicity and absorption tests. Lysis of bone marrow cells by the antiserum abolished granulocyte-macrophage colony formation in vitro. Absorption studies showed that myeloid colony forming cells express antigen common to T lymphocytes. The common antigen may be the membrane structure located close to the lymphocyte receptors for spontaneous binding of sheep erythrocytes. This common antigen probably represents an antigen characteristic of the T lymphocyte line which is shared with and thus may be inherited from the pluripotent stem cells.

Original languageEnglish (US)
Pages (from-to)6-15
Number of pages10
JournalExperimental Hematology
Volume8
Issue number1
StatePublished - 1980
Externally publishedYes

Fingerprint

T-Lymphocytes
Antigens
Immune Sera
B-Lymphocytes
Thymocytes
Granulocytes
Sheep
Erythrocytes
Lymphocytes
Rosette Formation
Pluripotent Stem Cells
Viral Tumor Antigens
Macaca mulatta
Goats
Bone Marrow Cells
Monocytes
Macrophages
Membranes
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Human T lymphocytes and myeloid colony forming cells share common antigen. / Chiao, J. W.; Pahwa, Rajendra; Good, R. A.

In: Experimental Hematology, Vol. 8, No. 1, 1980, p. 6-15.

Research output: Contribution to journalArticle

Chiao, JW, Pahwa, R & Good, RA 1980, 'Human T lymphocytes and myeloid colony forming cells share common antigen', Experimental Hematology, vol. 8, no. 1, pp. 6-15.
Chiao, J. W. ; Pahwa, Rajendra ; Good, R. A. / Human T lymphocytes and myeloid colony forming cells share common antigen. In: Experimental Hematology. 1980 ; Vol. 8, No. 1. pp. 6-15.
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